Nanopore-based sequencing platforms are transforming the field of genomic science. This review (containing 116 references) highlights some recent progress on nanopore-based nucleic acid analysis and sequencing. These studies are classified into three categories, biological, solid-state, and hybrid nanopores...
Ongoing developments promise further advances in the application of RNA-seq, particularly direct RNA sequencing and approaches that allow RNA quantification from very small amounts of cellular materials. This is a preview of subscription content, access via your institution Access options Subscription ...
Instead of isolating single cells within physical compartments, single-cell combinational indexing RNA sequencing (sci-RNA-seq) in 2017 was successively developed using a two-step combinatorial indexing strategy, a method using split-pool barcoding of nucleic acids to uniquely label a large number of ...
Instead of isolating single cells within physical compartments, single-cell combinational indexing RNA sequencing (sci-RNA-seq) in 2017 was successively developed using a two-step combinatorial indexing strategy, a method using split-pool barcoding of nucleic acids to uniquely label a large number of ...
To overcome this limitation, Wan and colleagues coupled NAI chemical probing with nanopore direct RNA sequencing to directly detect single-stranded positions along an RNA (Aw et al., 2021). Machine learning can be used to identify shifts in the current flowing through the pore when a base is ...
Lung cancer leads in causing cancer-related mortality worldwide, continually posing a significant threat to human health. Current imaging diagnostic techniques, while offering non-invasive detection, suffer from issues such as insufficient sensitivity an
Nevertheless, these are not intangibles and nanopore nucleic acid sequencing is envisaged to include methylation and direct RNA sequencing in the near future [83]. 4.4. NGS by electron microscopy The sequence of long, intact DNA molecules can be visualized and identified by using electron microscopy...
for DNA sequencing [56]. The plasmonic Au nanoparticle at the graphene nanopore leads to a multifold fluorescent signal enhancement in detecting DNA elongation during translocation through the nanopore (Figure 4C) [56]. Figure 4: Graphene nanopore with a self-integrated optical antenna. (A...
Advances in sequencing technology have greatly increased our ability to gather genomic data, yet understanding the impact of genetic mutations, particularly variants of uncertain significance (VUSs), remains a challenge in precision medicine. The CRISPR
In this context, various platforms are better suited for ctDNA sequencing, with Illumina sequencing by synthesis (SBS) being one of the prominent options, in addition to Ion Torrent and nanopore sequencing. The Illumina sequencing platform remains dominant due to its high throughput and precision. ...