如前所述,我们介绍了基于牛津纳米孔公司(Oxford Nanopore Technologies,ONT)三代测序平台进行全长转录组测序(full-length transcriptome sequencing,又称为Isoform Sequencing(Iso-Seq))。 研究背景 单个基因不同转录本isoform产生的蛋白质具有不同的生物特性,包括稳定性、细胞内定位、酶活性和翻译后修饰。Isoform是可选择...
Nano3P-seq:使用末端捕获Nanopore cDNA测序对基因表达和Poly(A)尾动力分析 文章题目:Nano3P-seq: transcriptome-wide analysis of gene expression and tail dynamics using end-capture nanopore cDNA sequencing 发表期刊:Nature Methods(IF=47.990) 发表时间:2022.12.19 研究内容 RNA 多聚腺苷酸化在 RNA 成熟、命运...
全长转录组,又称为Isoform Sequencing(Iso-Seq),无需打断,基于三代测序平台直接获取转录本的5ˊ到3ˊ高质量全长序列,可准确识别可变剪接、基因融合、可选择性多聚腺苷酸化APA、等位基因特异性表达等转录本结构方面变异。基于Nanopore三代测序平台进行全长转录组测序,除了可准确鉴别上述转录本结构变异,还可实现转录本...
为了评估ONT和Pacbio不同测序平台在植物转录组上的性能,我们选取植物模式物种拟南芥作为研究对象,对PacBio,Nanopore直接cDNA(ONT Dc)和Nanopore PCR cDNA(ONT Pc)测序的读数进行了详细评估,包括原始数据的特征和转录本的鉴定,同时与对应的Illumina数据进行比较。本文对拟南芥转录组的PacBio和Nanopore的RNA测序进行了全面比...
Here, we built a pipeline, UNAGI or UNAnnotated Gene Identifier, to process long reads obtained with nanopore sequencing and compared this pipeline with the standard Illumina pipeline by studying the Saccharomyces cerevisiae transcriptome in full-length cDNA samples generated from two different biological...
cDNA synthesis is initiated using random hexamers that amplifies the circRNA signal by forming concatemers of the same circRNA at the RT step and subjecting this to direct cDNA sequencing after library size selection. The average length distribution of the detected full-length circRNAs generated by ...
英文题目:Full-length Transcriptome Sequencing of Pepper Fruit during Development and Construction of a Transcript Variation Database 发表期刊:Horticulture Research 影响因子:7.6 发表日期:2024年7月 第一单位:湖南农业大学 参与单位:贝纳基因 背景介绍
文章题目:Aberrant splicing isoforms detected by full-length transcriptome sequencing as transcripts of potential neoantigens in non-small cell lung cancer 发表杂志:Genome Biology 影响因子:13.583 发表时间:2021.01.04 研究思路 主要结果 对肺癌...
et al. Improving nanopore read accuracy with the R2C2 method enables the sequencing of highly multiplexed full-length single-cell cDNA. Proc. Natl Acad. Sci. USA 115, 9726–9731 (2018). Article CAS PubMed PubMed Central Google Scholar Tang, A. D. et al. Full-length transcript ...
To identify and analyze endogenous targets of NMD, we apply cDNA Nanopore sequencing and short-read sequencing to human cells with varying expression levels of NMD factors. Our approach detects full-length NMD substrates that are highly unstable and increase in levels or even only appear when NMD...