Generate complete genome assemblies using long nanopore sequencing reads. Resolve structural variants, repeats, phasing, and base modifications.
测序技术的发展是人类对基因研究的转折点,使人类对基因组的研究从“盲人摸象”阶段走向更宽广与更精确的方向。全基因组重测序(Whole Genome Sequencing,WGS)是对已知基因组序列的物种进行不同个体的基因组测序,并在此基础上对个体或群体进行差异性分析。目前此技术已经广泛应用于医学领域,主要应用方向有寻找肿瘤突变位点...
Whole-genome sequencing (WGS) is becoming widely used in clinical medicine in diagnostic contexts and to inform treatment choice. Here we evaluate the potential of the Oxford Nanopore Technologies (ONT) MinION long-read sequencer for routine WGS by sequencing the reference sample NA12878 and the ...
12. Votintseva, A. A. et al. Sameday diagnostic and surveillance data for tuberculosis via whole genome sequencing of direct respiratory samples. J. Clin. Microbiol. JCM.02483-16 (2017). doi:10.1128/JCM.02483-16 13. Schmidt, K...
Whole genome sequencing and assembly of a Caenorhabditis elegans genome with complex genomic rearrangements using the MinION sequencing device 建库信息 (2D) ONT sequencing library(SQK-LSK108),上机4 MinION flowcells(R9.0)48hrs (1D) ONT sequencing library(SQK-RAD001),上机2 MinION flowcells(R9.3)48...
This application note highlights the potential of nanopore sequencing as a robust and standalone platform for whole-genome sequencing of foodborne pathogens, offering a promising approach to improving food safety. In this application note, you will: ...
Whole-Genome Sequence analysis of Clinically Isolated Carbapenem ResistantEscherichiacolifrom Iran (IRANCOLI ONT,SRR21721846,SRR21721848)。 #下载 MinION sequencing: IRANCOLI ONT, SRR21721846$ wget-c-t0ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR217/046/SRR21721846/SRR21721846_1.fastq.gz#数据大小521M...
Whole-genome sequencing (WGS) is becoming widely used in clinical medicine in diagnostic contexts and to inform treatment choice. Here we evaluate the potential of the Oxford Nanopore Technologies (ONT) MinION long-read sequencer for routine WGS by sequencing the reference sample NA12878 and the ge...
Fujimoto A, Wong JH, Yoshii Y,et al. Whole-genome sequencing with long reads reveals complex structure and origin of structural variation in human genetic variations and somatic mutations in cancer.Genome Med. 2021;13(1...
[1] Fujimoto A et al. Whole-genome sequencing with long reads reveals complex structure and origin of structural variation in human genetic variations and somatic mutations in cancer. Genome Medicine, 2021. 作者:胡潇雨,韩婉...