Nanopore的优势在于其超长的读长,在人类基因组中通过~30X reads和~5X的Ultra-Long reads,最长reads达到882Kb,组装出人类基因组contig N50达到6.4M(Jain et al., 2018)。但是Nanopore的同聚物错误使得这些错误往往出现在基因组某些特定的序列或区域,造成自身纠错和用二代数据校正无法纠正,序列错误和真实变异难以区分,...
记录每个孔的离子电流变化,并基于马尔可夫模型或递归神经网络的方法将其转换为碱基序列(图3)。其优势之一是支持RNA直接测序,除此之外,Ultra-long reads (ULRs) 是ONT平台的另一重要特征,并具有促进大型基因组组装的潜力。Nanopore测序平台相继推出MinION,GridION,PromethION及Flongle平台并投入使用(Table 2)。 图3 三...
Oxford Nanopore ultra-long read sequencingDisruptive innovations in long-range, cost-effective direct template nucleic acid sequencing are transforming clinical and diagnostic medicine. A multidrug resistant strain and a pan-susceptible strain of Mannheimia haemolytica, isolated from pneumonic bovine lung ...
Nanopore测序过程：解螺旋，将双链DNA解开成单链；DNA单链分子通过一个孔道蛋白，孔道中有个充当转换器的...
(NG50∼6.4 Mb). The final assembled genome was 2,867 million bases in size, covering 85.8% of the reference. Assembly accuracy, after incorporating complementary short-read sequencing data, exceeded 99.8%. Ultra-long reads enabled assembly and phasing of the 4-Mb major histocompatibility ...
近两年,nanopore领域发生的大事也很多,例如今年一月发表在『Nature Biotechnology』上的文章:Nanopore sequencing and assembly of a human genome with ultra-long reads 上图是1989记载了nanopore灵感的笔记本 3. nanopore产品 4. nanopore应用 研究领域: 潜在应用 ...
简评于武汉光谷 参考文献 [1] MJain, S Koren, J Quick, et al. Nanopore sequencing and assembly of a human genome with ultra-long reads. bioRxiv.2017©2022 Baidu |由 百度智能云 提供计算服务 | 使用百度前必读 | 文库协议 | 网站地图 | 百度营销...
Nanopore sequencing and assembly of a human genome with ultra-long reads. Nat. Biotechnol. 36, 338–345 (2018). CAS PubMed PubMed Central Google Scholar Wang, Y. Y., Zhao, Y., Bollas, A., Wang, Y. Y. & Au, K. F. Nanopore sequencing technology, bioinformatics and applications. ...
The most complete assemblies of large genomes Read N50s > 50kb, reads up to 4 Mbases Modified base information as standard Known and novel transcript ID Isoform detection Short fragment mode Standard modeUltra-long sequencingRNA sequencing
Read any length of DNA/RNA - short to ultra-long 超长读长 Scalable to portable or desktop 可扩展性,便携式或台式测序仪 Simple & rapid, or automated, library prep 10分钟文库制备 High yields for large genomes 对大基因组的高数据量测序