cDNA library enrichment of full length transcripts for SMRT long read sequencing. PLoS ONE 11, e0157779 (2016). Article PubMed PubMed Central Google Scholar Chen, Y. et al. A systematic benchmark of Nanopore long read RNA sequencing for transcript level analysis in human cell lines. ...
Mestre-Tomás, Jorge, et al. "SQANTI-SIM: a simulator of controlled transcript novelty for lrRNA-seq benchmark." Genome Biology 24.1 (2023): 286. Nanopore全长转录组测序
Learn how researchers are utilising nanopore sequencing to characterise all forms of human genomic variation, from repeat regions, SVs, and phasing to full-length transcript isoforms and methylation — delivering a comprehensive understanding of the human genome and genetic disease. Read white paper Comp...
Recommended device for fusion transcript sequencing MinION Delivering real-time sequencing anywhere, the portable MinION enables on-demand sequencing of full-length fusion transcripts. Targeted fusion or whole-transcriptom...
Tang, A. D. et al. Full-length transcript characterization of SF3B1 mutation in chronic lymphocytic leukemia reveals downregulation of retained introns.Nat. Commun.11, 1438 (2020). ArticleADSCASGoogle Scholar Gao, Y., Zhang, J. & Zhao, F. Circular RNA identification based on multiple seed ...
长链非编码RNA(Long noncoding RNA,Lnc RNA)是一类长度大于200碱基,且不具有编码蛋白质能力的RNA.Lnc RNA在真核生物细胞中广泛存在,近年研究表明,Lnc RNA能在细胞中以信号分子,分子诱骗物,蛋白质支架等方式参与细胞调控和细胞通信活动,且Lnc RNA被发现与肺癌,乳腺癌,胃癌等多种疾病息息相关.通过对Lnc RNA进行准确...
“Nanopore cDNA Sequencing Kits have provided us with quick workflows, full-length quantitative transcripts, and eased our data analysis since complex statistical considerations that need to be taken for short-read data, where full-length resolution of genes is difficult to determine, are not necessar...
Third-generation sequencing is able to read full-length transcripts and thus to efficiently identify RNA molecules and transcript isoforms, including transcript length and splice isoforms. In this study, we report the time-course profiling of the effect of bovine alphaherpesvirus type 1 on the gene ...
The gene finding tool AUGUSTUS is used to clean up these transcript projections and a combined gene set is generated62. To guide the annotation process, we obtained human RNA-seq data from SRA for a variety of tissues (Supplementary Table 7) and aligned them to both GRCh38 and the two ...
Figure 1. While legacy short-read sequencing techniques limit single-cell analysis to the gene level (A), nanopore sequencing of full-length transcripts provides isoform-level expression data (B). Image from Beaulaurier et al. (2024)1, reproduced with permission. Bringing together multiomic approac...