本研究表明,R10.4.1全长16S rRNA序列可用于环境微生物群的物种鉴定。 2023年10月6日,环境微生物领域业内权威期刊杂志Applied and Environmental Microbiology(简称AEM)在线发表了华中农业大学陈雯莉教授团队的研究成果,ONT 16S rRNA评测文章,文章题目为“The Newest Oxford Nanopore R10.4.1 Full-length 16S rRNA Seque...
[3] Mircea Cretu Stancu, et al. Mapping and phasing of structural variation in patient genomes using nanopore sequencing. Nat Commun . 2017 Nov 6;8(1):1326. [4]Alison D. Tang, et al. Full-length transcript characterization of SF3B1mutation in...
植物全长RNA图谱揭示了Poly(A)长度的组织特异性和进化保守性 文章题目:An atlas of plant full-length RNA reveals tissue-specific and monocots-dicots conserved regulation of Poly(A) tail length 发表期刊:Nature Plants(IF=17.352) 发表时间:2022.08.18 研究内容 Poly(A)尾是真核生物mRNA的标志之一,在调节m...
1.Cuscó A, Catozzi C, Viñes J et al. Microbiota profiling with long amplicons using Nanopore sequencing: full-length 16S rRNA gene and whole rrn operon 这篇文章采用了比较测16S和rrn序列(16S rRNA–ITS–23S rRNA; 4,500 bp),结果使用EPI2ME的话16S序列中只有68%的序列能够匹配到正确的分类。
Nanopore sequencing reveals full-length Tropomyosin 1 isoforms and their regulation by RNA-binding proteins during rat heart developmentalternative splicingheart developmentlong-read DNA sequencingRNA-binding proteinstropomyosinAlternative splicing (AS) contributes to the diversity of the proteome by producing ...
Our data also enabled a full-length sequence analysis of intron-containing circRNAs known as exon–intron circRNAs (EIciRNAs) in mouse and human brain, some of which were species specific (Supplementary Data5and6and Fig.3a, b). These RNAs have been reported to be localized in the nucleus an...
pychopper只有检测到双端的引物才能识别定义为全长完整(full length)的序列,所以在这之前不要对序列两端的引物(primers)进行裁剪去除,包括在用Dorado电信号转化碱基的时候。 通过-Q和-z可以实现chopper的功能,所以如果没有污染序列去除的话,可以跳过第二部直接使用pychopper。
et al. Translocation of linearized full-length proteins through an engineered nanopore under opposing electrophoretic force. Nat Biotechnol. https://doi.org/10.1038/s41587-023-01954-x (2023). Zhang, Y. et al. Peptide sequencing based on host–guest interaction-assisted nanopore sensing. Nat. ...
pychopper只有检测到双端的引物才能识别定义为全长完整(full length)的序列,所以在这之前不要对序列两端的引物(primers)进行裁剪去除,包括在用Dorado电信号转化碱基的时候。 通过-Q和-z可以实现chopper的功能,所以如果没有污染序列去除的话,可以跳过第二部直接使用pychopper。
Nonsense-mediated mRNA decay (NMD) is a eukaryotic, translation-dependent degradation pathway that targets mRNAs with premature termination codons and also regulates the expression of some mRNAs that encode full-length proteins. Although many genes express NMD-sensitive transcripts, identifying them based...