1 / 48In prokaryotes, transcription produces a nearly exact mRNA copy of the DNA, and the transcript is immediately translated into protein. In eukaryotes, a series of modificationsoccur to mRNA during and after transcription. 在原核生物中,转录产生的mRNA 几乎是DNA 的准确拷贝,并且这一转录产物会...
such as histone modification, pre-mRNA splicing and polyadenylation, RNA export, and stability control. Various evidence suggests that m6A addition takes place during transcription, which via its specific readers mediates coupling with other RNA
In eukaryotes, a series of modifications occur to mRNA during and after transcription. 在原核生物中,转录产生的mRNA几乎是DNA的准确拷贝,并且这一转录产物会立即被转译成蛋白质。在真核生物中,转录时以及转录后会对mRNA进行一系列修饰。 Chapter 5 mRNA Modifications in Eukaryotes 第5章 真核生物mRNA的修饰 ...
Compared to recombinant proteins expressed in mammalian cell lines, manufacturing of mRNA is faster and more flexible because mRNA can be easily produced by in vitro transcription. Over the past decade, technological discoveries in the areas of mRNA modifications and delivery systems have rapidly ...
poly(A) tail对维持mRNA的稳定性起着重要作用,体外合成mRNA常用的加尾方式有两种:(1)在IVT(in vitro transcription)后利用poly (A) polymerase进行加尾;(2)质粒模板上设计poly (A)序列,在IVT时被转录出来从而带上poly (A)尾,由于成本低,工艺简便,这...
Sequence-engineered mRNA without chemical nucleoside modifications enables an effective protein therapy in large animals. Mol. Ther. 23, 1456–1464 (2015). Article CAS PubMed PubMed Central Google Scholar Nelson, J. et al. Impact of mRNA chemistry and manufacturing process on innate immune ...
Synthesis and purification of three distinct mRNA types for mRNA-LNP drugs.AThe in vitro transcription (IVT) process is illustrated. First, a plasmid is generated containing the target gene with a T7 promoter. After restriction enzyme (RE) digestion of the plasmid and purification of linear DNA...
Types of synthetic mRNA for therapeutic application. A. Structural elements of mRNAs include the protein-encoding open reading frame (ORF), 5′ and 3′ untranslated regions (UTRs), 5′ cap structure, and 3′ poly (A) tail. mRNA drug design may involve several modifications to these structura...
transcription, which via its specific readers mediates coupling with other RNA metabolism pathways. Because intron removal and EJC deposition are known to take place co-transcriptionally, with the exception of various regulated exons,...
Modifications of mRNA vaccine structural elements for improving mRNA stability and translation efficiency. Mol. Cell. Toxicol. 2022, 18, 1–8. [Google Scholar] [CrossRef] [PubMed] Walsh, E.E.; Frenck, R.W., Jr.; Falsey, A.R.; Kitchin, N.; Absalon, J.; Gurtman, A.; Lockhart, ...