Quantification of mRNA in single cells and modelling of RT-qPCR induced noise BMC Mol Biol, 9 (2008), p. 63 CrossrefView in ScopusGoogle Scholar [30] J. Murphy, S.A. Bustin Reliability of real-time reverse-tran
mRNA 5′ untranslated regions (UTRs), which serve as the entry point for the ribosome during translation, can adopt elaborate RNA secondary and tertiary structures that may regulate translation initiation in a cap-dependent or cap-independent manner. Complex RNA structures in 5′ UTRs, such as ...
Ultraviolet (UV) radiation is a ubiquitous component of the environment that has important effects on a wide range of cell functions. Short-wavelength UVB radiation induces sunburn and is a potent immunomodulator, yet longer-wavelength, lower-energy UVA radiation also has effects on mammalian immunity...
Fig. 3. The m1ψ mRNA modification best enhanced in vivo luciferase expression for all delivery vehicles, with greatest improvement in the spleen. Mice were IV-injected with LNPs carrying either unmodified or modified luciferase mRNA at a dose of 0.75 mg/kg. Six hours after injection, organs ...
How is RNA converted into cDNA?cDNAcDNA refers to complementary DNA, and typically is synthesized from a mature mRNA template; that is, RNA that has had introns removed and a poly-A tail added to its 3' end. Often, the mRNA is isolated from a particular type of tissue or developmental ...
Through the process of transcription, the enzyme RNA polymerase synthesizes mRNA which contains the information for synthesis of a particular protein or small number of proteins. The mRNA is single-stranded and is antiparallel and complementary in nucleotide sequence...
Different from subunit vaccines, RNA-based vaccines work by introducing a mRNA encoding a disease-specific antigen. Once the sequence of interest is inside the cells, it serves as a template to produce the antigen (protein) in situ. After translation, the antigen could be extracellularly transpor...
Dmitriev, S. E. et al. Efficient translation initiation directed by the 900-nucleotide-long and GC-rich 5′ untranslated region of the human retrotransposon LINE-1 mRNA is strictly cap dependent rather than internal ribosome entry site mediated.Mol. Cell. Biol.27 ...
How are RNA bases freed up during transcription to form base pairs? a) How is DNA transcription different than DNA replication/synthesis? b) What is the role of messenger RNA (mRNA) during DNA transcription? c) What happens to the mRNA after transcription is complete ...
How is RNA converted into cDNA? How does small nuclear RNA help with the processing of RNA? What are deleted portions of an RNA transcript and how do they work? How does vesicular stomatitis virus regulate its mRNA transcription? Why can't double stranded RNA take part in the process of ...