mRNA Metabolism in Cardiac Development and Disease: Life After Transcriptiondoi:10.1152/PHYSREV.00007.2019Chen GaoYibin WangAmerican Physiological Society
As a retrovirus, HIV’s life cycle is marked by its single-stranded RNA genome, which replicates through reverse transcription and can integrate into the host genome1. Effective replication of HIV requires host cell activation, which leads to increased efficiency in reverse transcription, integration...
Lysis and barcoded reverse transcription of polyadenylated mRNA from single cells were performed inside each GEM followed by complementary DNA (cDNA) generation using the Single-Cell 3′ Reagent Kits version 2 (10X Genomics). Libraries were sequenced on an Illumina HiSeq 4000 as 2 × 100 ...
(B) Volcano plot with 9,978 genes tested in the differential expression, where yellow = upregulated genes; blue = downregulated genes. (C) We performed quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis of peripheral blood mononuclear cells gene expression at baseline and...
In contrast, the component explaining most of the variance was similar for the control and the urea treated cells, but differed on the second component. This indicates that the NaCl and raffinose treated cells had an overlapping transcription pattern. Of the genes regulated by NaCl, and not ...
Since emergence of the initial SARS-CoV-2 BA.1, BA.2 and BA.5 variants, Omicron has diversified substantially. Antigenic characterization of these new variants is important to analyze their potential immune escape from population immunity and implications for future vaccine composition. Here, we des...
Reverse transcription-PCR RNA from T1-CD19posand T2-CD19negsamples were extracted using RNAeasy mini kit (Qiagen), and bulk cDNA was synthesized using high capacity cDNA reverse transcription kit (Applied Biosystems). Then CD19 transcripts were assessed by PCR using specific primers (Supplementary...
Peripheral blood (2.0 ml) was collected from the ulnar vein of each participant before and after a plyometric exercise to assess HSPA1A and HSPB1 mRNA relative expression of leukocyte via quantitative reverse transcription polymerase chain reaction. Results A significant increment of leukocyte HSPA1A...
The conceptualization which framed molecular genetics studies for four decades is the so-called central dogma [36], representing the forward flow of gene expression from DNA to mRNA to proteins through transcription and translation. This directional flow can easily be viewed as an assembly line in ...
Most of the studies using 14 bp SAGE tags have focused on the expression of known genes. By contrast, here we concentrated on the tags that have not been generated by known transcripts. Because the main difficulty in estimating the amount of transcription in the human genome seems to be the...