To test the influence of shear forces on RNA quality or transcription, we compared the gene detection ability reflecting RNA integrity with different loading time. Our results suggested that at the loading time of 15 min and 40 min, the number of detected genes show no significant ...
1 / 48In prokaryotes, transcription produces a nearly exact mRNA copy of the DNA, and the transcript is immediately translated into protein. In eukaryotes, a series of modificationsoccur to mRNA during and after transcription. 在原核生物中,转录产生的mRNA 几乎是DNA 的准确拷贝,并且这一转录产物会...
Total RNA from cells in 6 well plates was extracted using a RNeasy Mini Kit (QIAGEN) and reverse-transcribed using a High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific). Primer sequences for humanGAPDHwere 5’-AGG GCT GCT TTT AAC TCT GGT-3’ and 5’-CCC CAC TTG ATT...
mRNA was produced using T7 High-Yield RNA Transcription kit (Novoprotein, Shanghai, China) on linearized plasmids. Then, Cap 1 was added to the synthesized RNA by using the Cap 1 Capping System (Novoprotein, Shanghai, China) and Pseudo-UTP (Ψ-UTP, APExBIO, Houston, TX, USA) was fully...
Reverse-transcription reaction components were gently mixed, briefly centrifuged (2000× g, 10 s), and kept on ice. The mixture was incubated at 37 °C for 60 min and then at 95 °C for 5 min in a Thermocycler (Thermo Fisher Scientific, San Francisco, CA, USA). After incubation, the...
Early transcripts are synthesized in abundance immediately after infection, reflecting the exceptional strength of most T4 early promoters. In fact, effective promoter competition for RNA polymerase can be considered one of the first mechanisms leading to shut-off of host gene transcription. Abundant and...
Using a subset of these mutants with translation efficiencies between 5% and 62%, we studied both physical and functional decay of the mRNA after rifampicin inhibition of transcription initiation. The decay of physically intact transcript was analyzed by gel electrophoresis of hybrid-selected messenger ...
Zheng WH, Long ZQ, Zheng ZQ, et al. m6A-enriched lncRNA LINC00839 promotes tumor progression by enhancing TAF15-mediated transcription of amine oxidase AOC1 in nasopharyngeal carcinoma. J Biol Chem. 2023;299:104873. Article CAS PubMed PubMed Central Google Scholar Download references ...
Nonstop mRNAs could be generated either by nuclease cleavages of an mRNA or by incomplete transcription. For example, they are produced as degradation intermediates from mRNAs by 3′-to-5′ exonucleolytic digestion (6). The tmRNA-mediated tagging of LacI typically represents the situation where ...
At 09.00, after the second blood sample, replaced milk was administered to the calves. Blood samples were immediately placed at 4deg;C until processing, which was performed on the same day. We used real-time reverse transcription PCR system to detect the expression of ghrelin mRNA levels in ...