Sleep deprivation impacts protein synthesis and gene regulation through many mechanisms including alterations to epigenetic regulation, transcription, and mRNA processing11,12,13,14,15,16,17,18,19. Estimates suggest that up to 10% of cortical transcripts are regulated with sleep/wake cycles, particular...
FFPE-stored tissue suffers RNA degradation from the 5′ end and yields limited amounts of RNA of acceptable quality [7]. Especially the chemical alteration of mRNA through formalin fixation can hamper reverse transcription to c-DNA and can consequently compromise the interpretation of RNA-sequencing...
Detecting prokaryotic mRNA, useful for gene expression profiling, includes reverse transcription after incorporation of RNA polymerase recognition site to form double-stranded cDNA for transcriptiondoi:DE10356751Detecting prokaryotic RNA (I) comprises providing (I); reverse transcription by introduction of an...
state or clustering of the population of PVN CRF cells in the developing mouse, we performed the same cluster analysis on control cells only and determined that segregation of PVN CRF cells into several biologically distinct clusters was not driven by ELA-induced changes to transcription (Figure ...
The samples were left to cool down to room temperature. Fifty units of reverse transcriptase, 20 units of recombinant human placenta RNase inhibitor, 50 μM dNTPs and 50 μM DTT were added. The reverse transcription was carried out for 1 h at 42 °C. The resulting cDNA was diluted 1:5...
(CDI). CDI is of necessity linked to DNA replication, DNA repair, and transcription, but must be considered in any nucleosomal histone turnover model. Because it is hard to imagine a complete physical mechanism for the CDI of properly modified nucleosomes following replication or transcription, ...
2.6.2. MicroRNA Reverse-Transcription Quantitative PCR Analysis in Serum RNA (RT-qPCR) Starting from total RNA samples, cDNA was synthesized using the miRCURY LNA RT kit (Qiagen, Hilden, Germany). Reaction conditions were set in accordance with the manufacturer’s specifications. In total, 2 ...
The cascade is relayed by Gli family proteins (Gli1, Gli2, etc.). This pathway is inhibited by another 7-transmembrane protein Ptch1 and is released when it binds to ligand Shh. The regulation of gene transcription results from the translocation of Gli family proteins to the nucleus [80]....
and then review the status of survivin cancer therapeutics, which is classified into five categories: (i) survivin-partner protein interaction inhibitors, (ii) survivin homodimerization inhibitors, (iii) survivin gene transcription inhibitors, (iv) survivin mRNA inhibitors and (v) survivin immunotherapy...
(e.g. histones and transcription factors) to regulate, among others, DNA structure, repair and transcription [72,73]. HMGB1 is released in response to pathogen-associated molecular patterns or cellular stress (e.g. stroke) and functions as a pro-inflammatory cytokine [74,75]. It translocates...