1993. Reverse transcription of mRNA by Thermus aquaticus DNA polymerase followed by polymerase chain reaction amplification. Methods Enzymol. 218:413-419.Jones, M. D., and N. S. Foulkes. 1989. Reverse transcription of mRNA by Thermus aquaticus DNA polymerase. Nucleic Acids Res. 17:8387-8388....
Reverse Transcription of mRNA The success of the differential display technique is dependent on the integrity of the RNA and that it is free of chromosomal DNA contamination. Upon completion of DNase-I treatment of the RNA, the mRNA then can be reverse transcribed into cDNA. 1. Set up three...
Reverse transcription-polymerase chain reaction (RT-PCR) is a powerful method to detect and synthesize cDNA copies of low-copy-number mRNAs. Two enzymes are used: reverse transcriptase to produce single-stranded cDNA copies, which are then used as templates in an amplification reaction catalyzed by...
3.TLR 4 mRNA in KCs was determined by the reverse transcription polymerase chain reaction ( RT - PCR ) 用逆转录多聚酶联反应 ( RT -PCR ) 测定KCs中TLR4mRNA的表达. 4.The gene expression of ET - 1、 ETAR 、 ETBR and ECE was evaluated by semi - quantitative reverse transcription polymerase...
RT-PCR (Reverse transcription PCR) 实验目的:学习RT-PCR的原理及其操作过程。 实验材料:水稻叶片的RNA。 实验原理:目前PCR技术只能扩增DNA模板,对 RNA模板不能直接扩增。mRNA 反转录生成的cDNA可作为PCR的模板进行扩增,这种在mRNA反转录后进行的PCR扩增称为RT-PCR。RT- PC...
mRNA for four different genes was reverse-transcribed at different temperatures using oligo (dT)20 (12.3 kb mRNA) or gene-specific primers (9.3 kb, 7 kb, and 5.5 kb mRNAs). Reverse transcription at 55°C generated higher specificity of the ...
Visit ChemicalBook for more information on MRNA Reverse Transcription Optimization Kit, including its chemical and physical properties, structure, melting point, boiling point, density, molecular formula, molecular weight, uses, prices, suppliers, and sa
Reverse transcription-polymerase chain reaction quantitation revealed that receptor mRNA was differentially expressed during the estrous cycle. Although, mRNA levels for both receptor types increased steadily up to Day 4:0900 h, a sharp ... SK Roy - 《Biology of Reproduction》 被引量: 0发表: 2000...
Background Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is the gold standard technique for mRNA quantification, but appropriate normalization is required to obtain reliable data. Normalization to accurately quantitated RNA has been proposed as the most reliable method for in vivo...
A reverse transcription (RT) and competitive polymerase chain reaction (PCR) amplification technique was developed to quantify bovine tumor necrosis factor-alpha (TNF-α) mRNA. A bovine TNF-α cDNA insert containing plasmid was used to produce a 237-bp cDNA competitive template which, when PCR-am...