因此，从LNP 中提取 mRNA进行完整性检测是必要的质控手段。2024年1月，赛诺菲(Sanofi)团队Camille Malburet等人在Journal of Chromatography A发表文章：《mRNA extraction from lipid nanoparticles》，从 mRNA 提取率和 mRNA 完整性出发，他们比较了从LNP中提取mRNA的不同方法的效果。除了这种定量评估之外，还讨论了其他...
2024年1月,赛诺菲(Sanofi)团队Camille Malburet等人在Journal of Chromatography A发表文章:《mRNA extraction from lipid nanoparticles》,从 mRNA 提取率和 mRNA 完整性出发,他们比较了从LNP中提取mRNA的不同方法的效果。除了这种定量评估之外,还讨论了其他定性因素,例如,提取方法自动化的可能性和有毒试剂的使用。 一...
参考资料:1. Automated mRNA extraction from lipid nanoparticles to enable purity analyses.https://acs.digitellinc.com/p/s/automated-mrna-extraction-from-lipid-nanoparticles-to-enable-purity-analyses-6042582. Automated mRNA Extraction from Lipid Nanoparticles Using the SOTAX TPW. https://www.sotax.com/...
因此,需要进一步改进LNPs的内体逃逸效率和生物安全性,以更好地利用mRNA的潜能。 2024年3月7日,浙江大学顾臻团队在期刊《ACS Nano》发表了一篇题为"Fluorinated Lipid Nanoparticles for Enhancing mRNA Delivery Efficiency"的研究文章,开发了一种通过氟化修饰PEG来...
To function in vivo, mRNA requires safe, effective and stable delivery systems that protect the nucleic acid from degradation and that allow cellular uptake and mRNA release. Lipid nanoparticles have successfully entered the clinic for the delivery of mRNA; in particular, lipid nanoparticle–mRNA ...
ALC-0315、MC3、和SM102是三种可用于人体的可解离脂质,在酸性条件下可质子化形成阳离子脂质,通过静电作用和带负电的mRNA结合,形成载有mRNA的脂质纳米颗粒(lipid nanoparticles, LNPs)。本实验中采用微流控混合法,让脂质溶液与和mRNA溶液在微混合器中充分、迅速的形成粒径均一的LNP。由于脂质溶解于乙醇中,核酸溶解于...
effective and stable delivery systems that protect the nucleic acid from degradation and that allow cellular uptake and mRNA release. Lipid nanoparticles have successfully entered the clinic for the delivery of mRNA; in particular, lipid nanoparticle–mRNA vaccines are now in clinical use against corona...
Lipid nanoparticles for the inhalation of mRNARachel Yoon Kyung Chang & Hak-Kim Chan Nature Biomedical Engineering volume 5, pages 949–950 (2021)Cite this article 15k Accesses 46 Altmetric Metrics details Lipid nanoparticles can be optimized for the efficient delivery of mRNA via nebulization. This...
[2] Daniel Rosenblum et al., CRISPR-Cas9 genome editing using targeted lipid nanoparticles for cancer therapy. Sci.Adv.6, eabc9450(2020). [3] Mirjalili Mohanna SZ, Djaksigulova D, Hill AM, Wagner PK, Simpson EM, Leavitt BR. LNP-mediated delivery of CRISPR RNP for wide-spread in vivo...
LipidNanoparticles FromLiposomestomRNAVaccineDeliveryaLandscapeofResearchDiversityandAdvancementRumianaTenchovRobertBirdAllisonE.CurtzeandQiongqiongZhou*CiteThis:https://doi.org/10.101/acsnano.1c04996ReadOnlineACCESSMetrics&MoreArticleRecommendations*s