因此，从LNP 中提取 mRNA进行完整性检测是必要的质控手段。2024年1月，赛诺菲(Sanofi)团队Camille Malburet等人在Journal of Chromatography A发表文章：《mRNA extraction from lipid nanoparticles》，从 mRNA 提取率和 mRNA 完整性出发，他们比较了从LNP中提取mRNA的不同方法的效果。除了这种定量评估之外，还讨论了其他...
因此,从LNP 中提取 mRNA进行完整性检测是必要的质控手段。 2024年1月,赛诺菲(Sanofi)团队Camille Malburet等人在Journal of Chromatography A发表文章:《mRNA extraction from lipid nanoparticles》,从 mRNA 提取率和 mRNA 完整性出发,他们比较了从LNP中提取mRNA的不同方法的效果。除了这种定量评估之外,还讨论了其他定...
参考资料:1. Automated mRNA extraction from lipid nanoparticles to enable purity analyses.https://acs.digitellinc.com/p/s/automated-mrna-extraction-from-lipid-nanoparticles-to-enable-purity-analyses-6042582. Automated mRNA Extraction from Lipid Nanoparticles Using the SOTAX TPW. https://www.sotax.com/...
Lung-selective mRNA delivery of synthetic lipid nanoparticles for the treatment of pulmonary lymphangioleiomyomatosis 原文链接:https://doi.org/10.1073/pnas.2116271119 Significance 目前基于mRNA技术的应用主要局限于肝脏疾病,因为缺乏特异性和高效的肝外的体内mRNA递送系统。在这里,我们开发了一个n系列脂质纳米颗粒...
To function in vivo, mRNA requires safe, effective and stable delivery systems that protect the nucleic acid from degradation and that allow cellular uptake and mRNA release. Lipid nanoparticles have successfully entered the clinic for the delivery of mRNA; in particular, lipid nanoparticle–mRNA ...
effective and stable delivery systems that protect the nucleic acid from degradation and that allow cellular uptake and mRNA release. Lipid nanoparticles have successfully entered the clinic for the delivery of mRNA; in particular, lipid nanoparticle–mRNA vaccines are now in clinical use against corona...
Lipid nanoparticles for the inhalation of mRNARachel Yoon Kyung Chang & Hak-Kim Chan Nature Biomedical Engineering volume 5, pages 949–950 (2021)Cite this article 15k Accesses 46 Altmetric Metrics details Lipid nanoparticles can be optimized for the efficient delivery of mRNA via nebulization. This...
blood–brain barrierlipid nanoparticlemRNALipid nanoparticles (LNPs) have delivered RNA to hepatocytes in patients after intravenous administration. These clinical data support efforts to design LNPs that transfect cells in the central nervous system (CNS). However, delivery to the CNS has been ...
[2] Daniel Rosenblum et al., CRISPR-Cas9 genome editing using targeted lipid nanoparticles for cancer therapy. Sci.Adv.6, eabc9450(2020). [3] Mirjalili Mohanna SZ, Djaksigulova D, Hill AM, Wagner PK, Simpson EM, Leavitt BR. LNP-mediated delivery of CRISPR RNP for wide-spread in vivo...
To function in vivo, mRNA requires safe, effective and stable delivery systems that protect the nucleic acid from degradation and that allow cellular uptake and mRNA release. Lipid nanoparticles have successfully entered the clinic for the delivery of mRNA; in particular, lipid nanoparticle–mRNA ...