LNP组成 脂质纳米颗粒(Lipid Nanoparticle,LNP) 是目前主流的载体递送方式,现阶段主要的mRNA疫苗都使用的是此项技术。LNP是一种多组分系统,通常由可电离脂质 (lonizable lipid) 、辅助脂质(Helper lipid) 、胆固醇 (cholesterol) 和聚乙二醇修饰脂质 (PEG-lipid) 组成,它们分工合作,将mRNA包裹在核中以避免降解,帮助...
A drawback of the current mRNA-lipid nanoparticle (LNP) COVID-19 vaccines is that they have to be stored at (ultra)low temperatures. Understanding the root cause of the instability of these vaccines may help to rationally improve mRNA-LNP product stability and thereby ease the temperature condi...
In addition, the study demonstrates a novel method of delivering bispecific antibodies using mRNA-lipid nanoparticle (LNP) technology. The delivery of EpCAM-CD3 human Fc (hFc) mRNA-LNPs into mice tumors with intravenous T cell injection significantly blocked OVCAR-5 xenograft...
Lipid nanoparticle–mRNA formulations manufactured by rapid mixing exhibit a stable nanostructure17,130,131, in which mRNA molecules can be encapsulated in the interior core through electrostatic interactions with the lipids17,131. This structural feature protects mRNA molecules from nuclease degradation and...
J. Inside out: optimization of lipid nanoparticle formulations for exterior complexation and in vivo delivery of saRNA. Gene Ther. 26, 363–372 (2019). CAS Google Scholar Lou, G. et al. Delivery of self-amplifying mRNA vaccines by cationic lipid nanoparticles: The impact of cationic lipid ...
. The payload distribution and capacity of mRNA LNPs are important characteristics to assess, because they hint at molecular assembly mechanisms and influence pharmacodynamics, pharmacokinetics, and delivery efficiency6,11,12. However, Cryo-TEM, and other common nanoparticle characterization methods such ...
不久前,国际知名mRNA药物巨头Moderna在对Arbutus主动发起的LNP(脂质纳米粒,Lipid Nanoparticle)专利挑战中再度败诉。法院仍认定Arbutus的“435专利部分有效”、“069专利完全有效”。 这一判决结果公布后,Moderna股价直线下挫,而Arbutus盘中股价上涨一度接近翻倍。
In a mouse model, PTPN14 mRNA‐lipid nanoparticle delivery has reduced tumor growth and metastasis, highlighting its therapeutic potential. 展开 关键词: anoikis resistance cancer therapy mRNA therapeutics PTPN14 triple‐negative breast cancer DOI: 10.1002/advs.202309988 年份: 2024 ...
This report is intended to serve as a useful resource for those interested in LNPnanotechnologies, their applications, and the global research ef f ort for their development.KEYWORDS: lipid nanoparticle, liposome, cationic lipid, solid lipid nanoparticle, nanostructured lipid carrier, immunoliposome,“...
Intratumoral delivery of lipid nanoparticle-formulated mRNA encoding IL-21, IL-7, and 4-1BBL induces systemic anti-tumor immunity Article Open access 06 December 2024 Cancer nanomedicine meets immunotherapy: opportunities and challenges Article 17 June 2020 Nucleic acid-based drugs for patients ...