人的经典单核细胞表现为CD14高表达，但缺乏 CD16，记为CD14++ CD16-。CD14 是细菌脂多糖的共同受体，在单核细胞和巨噬细胞中特异性表达。CD16 是一种抗体 (IgG) 受体，由两个几乎相同的基因FCGR3A和FCGR3B表达。另外，在人的经典单核细胞中还存在高水平的CCR2、CCR5和CD62L。2、非经典单核细胞 中级单核...
CD14++CD16+monocyte percentage was significantly higher in long standing RA patients compared with early patients and controls (p<0.01,p<0.001 respectively). It was significantly higher in patients with RA disease activity and remission compared with the controls (p<0.001,p<0.01 respectively). It ...
CD14 是细菌脂多糖的共同受体,在单核细胞和巨噬细胞中特异性表达。CD16 是一种抗体 (IgG) 受体,由两个几乎相同的基因FCGR3A和FCGR3B表达。另外,在人的经典单核细胞中还存在高水平的CCR2、CCR5和CD62L。 2、非经典单核细胞 中级单核细胞(Intermediate monocyte)表现为高表达CD14和低表达CD16,记为CD14++ CD16...
人经典单核细胞分离试剂盒可快速,一致地从外周血单核细胞(PBMC)中分离未经标记的经典(CD14 ++ CD16-)单核细胞,以进行进一步的功能研究。 对非单核细胞进行了最佳的去除并且提供高纯度的未经标记的经典单核细胞群。 该产品包括可选的血小板去除试剂,以进一步减少血小板污染。
起始样本为新鲜制备的外周血单核细胞,富集后CD14+细胞纯度通常为73%-81%。 可从含有大量CD16+细胞的样品获得稍低纯度的CD14+细胞。 组成成分: ▪EasySep™人单核细胞富集抗体混合物,不去除CD16,1 mL ▪EasySep™磁珠,1 mL ▪ RoboSep™缓冲液*(货号#20104) ...
起始样本为新鲜制备的外周血单核细胞,富集后CD14+细胞纯度通常为73%-81%。 可从含有大量CD16+细胞的样品获得稍低纯度的CD14+细胞。 组成成分: ▪EasySep™人单核细胞富集抗体混合物,不去除CD16,1 mL ▪EasySep™磁珠,1 mL ▪ RoboSep™缓冲液*(货号#20104) ...
Cells are first gated on TLR2 expression, monocytes (TLR2+) are further gated by CD14 and CD16 expression, classical (CD14 + CD16−), intermediate (CD14 + CD16+) and non-classical (CD14-CD16+). Lymphocytes (TLR2-) are gate based on CD3 and CD4 expression, CD4 T cells ...
Based on the difference in the CD14 and CD16 expression, two subsets of monocytes were identified in human and other mammalian blood. These subsets have different patterns of adhesion molecules and chemokine receptors that suggests the different mode of their interaction with endothelium and tissue tr...
MagniSort™ Human pan-Monocyte Enrichment Kit 设计通过磁性阴性分选分离常规 CD14+ 和非常规 CD16+ CD14- 单核细胞。它经过优化,可利用生物素化的抗体混合物和链霉亲和素包被的磁珠从正常人类外周血单核细胞中分离人类泛单核细胞。非目标细胞(包括 CD16+ NK 细胞)将由抗体结合,随后结合至磁珠,将其放入磁力...
Objective Circulating monocytes contain a subpopulation of CD14+CD16+ cells; this subpopulation of cells has been described to be proinflammatory and to have an increased frequency in rheumatoid arthritis (RA). New evidence suggests that this subpopulation can be further subdivided into CD14 dim CD16...