CX3CR1 dose-dependently modulated monocyte-contact-dependent gene expression in human endothelium.在肾功能衰竭患者中,移植后一年内,CD16 单核细胞计数升高,并动态下降,主要原因是CD14 CD16 细胞减少。CX3CR1配体CX3CL1在患...
Human umbilical vein endothelial cells (HUVECs) differentiate human CD14highCD16−monocytes into CD14+CD16+monocytes along with high expression of CX3CR1. (a) Specific monocyte subsets were separated in human liver MNCs and peripheral blood mononuclear cells (PBMCs). (b,c) Human CD14highCD16...
Human CD14dim monocytes patrol and sense nucleic acids and viruses via TLR7 and TLR8 receptors. Immunity 33, 375–386 (2010). Article CAS PubMed PubMed Central Google Scholar Ellery, P.J. et al. The CD16+ monocyte subset is more permissive to infection and preferentially harbors HIV-1 ...
Multicolor flow cytometry was performed on thawed PBMC samples using an LSRFortessa (BD). Samples were stained using the following fluorochrome-conjugated antibodies: PE-CF594 mouse anti-human CD14 (BD), APC mouse anti-human CD16 (BD), BV 421 mouse anti-human CD56 (BioLegend), and PE rat...
CD14 BUV395 BD Biosciences catalog number 563561, RRID:AB_2744288 CD56 BUV737 BD Biosciences catalog number 564447, RRID:AB_2744432 CD19 BUV496 BD Biosciences catalog number 564655, RRID:AB_2744311 CD45 BUV805 BD Biosciences catalog number 564914, RRID:AB_2744401 EGFR AF488 R&D Systems catalo...
Background: Peripheral blood monocytes can be subdivided into different subsets based on the CD14/CD16 surface characteristics. Monocytes are a major source of cytokine secretion of pro-inflammatory immune responses, whereas CD16 monocyte subsets can also contribute to persistent inflammation in the ...
Both functions are governed by the distribution of CX3CR1, a specific seven transmembrane domain G protein-coupled receptor for fractalkine expressed on human peripheral blood CD8+ (12) and CD4+ perforin+ T cells (12, 13), CD56dim CD16+ NK cells, NK-T cells (12, 14), and CD14lowCD...
. Human counterparts of Ly6Chighand Ly6Clowmonocytes are defined as CD14++/CD16−and CD14+/CD16++[175,176]. The main source of knowledge regarding the direct functioning of the CX3CL1/CX3CR1 axis in TBI and SCI is studies on animal models which were subjected to substitution on one ...
Similarly, increased circulating CD16+ monocytes levels correlated with vulnerable plaque as measured by multidetector computed tomography in patients with stable angina pectoris (Kashiwagii, 2010; Atherosclerosis 212:71) and CD14+CD16+ monocyte levels independently predicted cardiovascular events in patients...
Differential expression of CCR2 and CX3CR1 on CD16+ monocyte subsets is associated with asthma severitySaudi ArabiaChemokine receptorFlow cytometryCD14BiomarkerDemographic and clinical characteristics of subjects with asthma and healthy controlsdoi:10.1186/s13223-019-0379-5Reem Al-Rashoudi...