CX3CR1 dose-dependently modulated monocyte-contact-dependent gene expression in human endothelium.在肾功能衰竭患者中,移植后一年内,CD16 单核细胞计数升高,并动态下降,主要原因是CD14 CD16 细胞减少。CX3CR1配体CX3CL1在患有...
Human umbilical vein endothelial cells (HUVECs) differentiate human CD14highCD16−monocytes into CD14+CD16+monocytes along with high expression of CX3CR1. (a) Specific monocyte subsets were separated in human liver MNCs and peripheral blood mononuclear cells (PBMCs). (b,c) Human CD14highCD16...
GMA effectively depletes CD14 + CD16 + monocytes and concomitantly increases CD14 hi CD16 CCR2 low "immature" monocytes; thus GMA was associated with the emergence of less inflammatory monocyte phenotype in circulation. 展开 关键词: Ulcerative colitis CD14+CD16+ monocytes CD14hiCD16−CCR2low ...
Human CD14dim monocytes patrol and sense nucleic acids and viruses via TLR7 and TLR8 receptors. Immunity 33, 375–386 (2010). Article CAS PubMed PubMed Central Google Scholar Ellery, P.J. et al. The CD16+ monocyte subset is more permissive to infection and preferentially harbors HIV-1 ...
. Human counterparts of Ly6Chighand Ly6Clowmonocytes are defined as CD14++/CD16−and CD14+/CD16++[175,176]. The main source of knowledge regarding the direct functioning of the CX3CL1/CX3CR1 axis in TBI and SCI is studies on animal models which were subjected to substitution on one ...
(iv) finally, monocytes were gated based on their high side scatter (SSC-A) and CD14 positivity. Monocyte subtypes were gated from a starting population of all monocytes based on their characteristic CD14 vs. CD16 staining patterns. For quantifications, frequencies expressed as a percentage of ...
Differential expression of CCR2 and CX3CR1 on CD16+ monocyte subsets is associated with asthma severitySaudi ArabiaChemokine receptorFlow cytometryCD14BiomarkerDemographic and clinical characteristics of subjects with asthma and healthy controlsdoi:10.1186/s13223-019-0379-5Reem Al-Rashoudi...
Background: Peripheral blood monocytes can be subdivided into different subsets based on the CD14/CD16 surface characteristics. Monocytes are a major source of cytokine secretion of pro-inflammatory immune responses, whereas CD16 monocyte subsets can also contribute to persistent inflammation in the ...
Based on specific membrane markers and functional features, HDCs can be distinguished into plasmacytoid and myeloid or classical subsets. Myeloid HDCs are further sub-grouped in type-1 (CD103+/CD11b− in mice; CD141+/CD14− in humans) and type-2 (CD103−/CD11b+ in mice; CD1c+/...
but not inCcr2RFPreporter mice. In conclusion, our data suggest that phenotyping strategies for mouse monocyte subsets should be carefully selected. In accordance with the literature, the suitability of CX3CR1 antibody staining is limited, whereas for CCR2, caution should be applied when using re...