Another Fc receptor (FcR) that is differentially expressed by this monocyte subset is the Fcγ receptor I (CD64). This Fcγ receptor was found to be expressed on the majority of monocytes, so the major subset C
The proportion of monocyte was estimated by CD14/CD64 gating and compared with those using CD45/LS gating. The type of PNH clone was defined according to the size of PNH population. A PNH clone is defined as a PNH population exceeding 1% of the gated cells, a minor PNH clone as ...
Normal human peripheral blood cells were stained with CD14 Monoclonal Antibody, FITC (Product # 11-0149-42) and Mouse IgG1 kappa Isotype Control, PE-Cyanine7 (Product # 25-4714-80) (left) or CD64 Monoclonal Antibody, PE-Cyanine7 (right). Cells in the monocyte gate were used for analysis...
Normal human peripheral blood cells were stained with CD14 Monoclonal Antibody, FITC (Product #11-0149-42) and Mouse IgG1 kappa Isotype Control, PE-eFluor 610 (Product #61-4714-82) (left) or CD64 Monoclonal Antibody, PE-eFluor 610 (right). Cells in the monocyte gate w...
Expression of CD64 on differentiating myeloid cells. The expression of CD64 during the later stages of granulocyte and monocyte maturation was investigated in costaining experiments with MoAbs to CD14 and CD11b, which are upregulated during myeloid differentiation34, 35, 36 (Fig 6). Figure 6 sho...
Conventional GMPs (cGMPs) represent the most restricted subset of myeloid progenitors in humans13,14. Based on flow cytometry approaches15, cGMPs have been fractionated into three main compartments: granulocyte-monocyte-dendritic cell (DC) progenitors (GMDPs), which generate CD66b+granulocytes, CD14+...
This review will focus on CD64 as an attractive target for treatment of chronic inflammatory diseases mediated by dysfunctional M1-type pro-inflammatory macrophages. 2. CD64 as A Therapeutic Target for M1 Dysregulated Macrophages in Chronic Inflammatory Diseases The cells of the monocyte phagocytic ...
Evidence on the changes in the absolute counts of monocyte subpopulations in sepsis is missing. 背景 目前关于脓毒症患者外周血中单核细胞亚群绝对计数的证据是缺失的。 Methods Firstly, absolute counts of circulating CD14pos...
Flow cytometry analysis comprised monocytic and granulocytic surface expression of CD14, CD16, CD64, TLR2, TLR4 and Dectin-1 (CLEC7A). Monocyte subpopulations were further defined as classical (CD14/CD16), intermediate (CD14/CD16) and nonclassical (CD14/CD16) monocytes. Plasma concentrations of ...
Circulating CD14+CD16+ and CD14+CD163+ monocytes were elevated in No-SIL and cervical lesions. Interestingly, CD14+CD64+ monocyte was greatly elevated in HSIL and SCC, whereas intracellular IL-10+ monocytes were not significantly different between cervical lesions. The correlation between increasing...