Flow cytometry was used to quantitate CD14, HLA-DR, and CD16 marker expression on monocyte subpopulations and neutrophils, as well as their cell population frequencies. A Generalized Estimating Equation model was used to assess the association between changes in these immune parameters and disease ...
(low))2例,且均表达CD13和CD33,不表达CD4,而CD34,CD38,CD117,CD15,CD64,CD14和CD56均呈异质性表达;与HLA-DR^(posi)阳性者相比,HLA-DR^(neg)/low者CD4的阳性率差异有统计学意义(P=0.015),其他抗原阳性率的差异均无统计学意义.结论 HLA-DR^(neg/low)的AML-M5患者CD抗原表达异常,多呈HLA-D...
HLA-DR是由36 kDa α链和27 kDa β链组成的异二聚体,由6号染色体短臂的MHC基因复合物编码。两条链可由许多不同的等位变异形态出现,存在高度的多态性。HLA-DR结构性表达在抗原递呈细胞上,主要表达于免疫系统的细胞[1]。HLA-DR在单核细胞上强表达,尤其是CD14+CD16+亚群[2];在巨噬细胞、粒细胞、浆样树突...
Regulatory T cells: CD3, CD4, CD127, CD25 B cells: CD19, CD20, CD38, CD27 Natural killer (NK) cells and NKT cells: CD56, CD3, CD16 Activation markers: CD69, HLA-DR Monocytes and dendritic cells (DCs): CD14, CD16, HLA-DR, CD11b, CD11c, CD209, CD86 We can also include a ‘...
The human syndrome of dendritic cell, monocyte, B and NK lymphoid deficiency HLA-DR(+) lineage(-) compartment, with virtually no CD123(+) or CD11c(+) dendritic cells (DCs) and very few CD14(+) or CD16(+) monocytes. The... Venetia,Bigley,Muzlifah,... - 《Journal of Experimental ...
HLA-DR expression was decreased on each subtype of monocyte of NHL patients, among which the difference of intermediate monocyte (CD14++CD16+) (25.43 MFI vs. 33.52 MFI, P<0.01) and non-classical monocyte (CD14+CD16++) (11.40 MFI vs. 15.70 MFI, P<0.01) were of statistical significance...
CD14, CD36 and CD64 are considered as monocyte-associated markers, CD14, the lipopolysaccharide receptor, being the most specific. During maturation towards promonocytes, monoblasts down-regulate CD34 and CD117 and gain expression of CD64, CD33, HLA-DR, CD36 and CD15 antigens, with an initia...
目的:观察多器官功能障碍综合征(MODS)患者的免疫功能改变,寻求早期诊断手段.方法:采用免疫荧光技术连续监测28例ICU中重症感染、严重创伤患者的外周血单核细胞表面人类白细胞抗原DR位点(HLA-DR)抗原表达.结果:发生MODS患者HLA-DR抗原表达水平比未发生MODS者显著降低(P<0.05) ,而且其动态变化与预后有明显的相关性.结论...
Myeloid derived suppressor cells are present and predominantly localized in the ONB stroma To identify myeloid cells in the ONB TIME a mxIF panel of C11b, HLA-DR, CD14, CD15, CD68, and synaptophysin was used (Fig. 2A). Recent studies have shown that CD68+ is overexpressed in tumor asso...
(cat. 555432), CD127 (cat. 560549), CD8 (cat. 347314), CD19 (cat. 340865), CD16 (cat. 338440) and CD14 (cat. 340660) antigens. The last 4 mAbs were conjugated with the same fluorochrome with the aim to cut-off in one step cytotoxic T cells (Tc), B cells, NK cells and ...