Monocyte subset composition was determined by flow cytometry based on the expression of CD14, CD16, and HLA-DR. Monocyte subset composition and the expression of CD14 and HLA-DR were correlated with patient outcomes. The results were compared to 19 patients with coronary artery disease. Cardiac ...
HLA-DR是由36 kDa α链和27 kDa β链组成的异二聚体,由6号染色体短臂的MHC基因复合物编码。两条链可由许多不同的等位变异形态出现,存在高度的多态性。HLA-DR结构性表达在抗原递呈细胞上,主要表达于免疫系统的细胞[1]。HLA-DR在单核细胞上强表达,尤其是CD14+CD16+亚群[2];在巨噬细胞、粒细胞、浆样树突...
急性脑梗死患者单核细胞CD14+CD16+亚型的表达变化及临床意义 热度: 皮肌炎患者外周血单核细胞PD-L1分子的表达及其临床意义① 热度: ·临床研究· HLA-DR / CD14 在外周血单核细胞中的表达及其临床意义 李薇,谢闻悦,张卫云,张璇,张玉新,高贵阳 [摘要] ...
CD抗原及其相应的单克隆抗体在基础和临床免疫学研究中已得到广泛的应用,在基础免疫学研究中CD主要应用于:①CD抗原的基因克隆,新CD抗源及新配体的发现;②CD抗原结构与功能关系;③细胞激活途径和膜信号的传导;④细胞分化过程中的调控;⑤细胞亚群的功能...
Myeloid derived suppressor cells are present and predominantly localized in the ONB stroma To identify myeloid cells in the ONB TIME a mxIF panel of C11b, HLA-DR, CD14, CD15, CD68, and synaptophysin was used (Fig. 2A). Recent studies have shown that CD68+ is overexpressed in tumor asso...
anti-Human CD14 (Mouse monoclonal) BioLegend Cat# 301859; Clone M5E2; RRID:AB_2800736 anti-Human CD16 (Mouse monoclonal) BioLegend Cat# 302065; Clone 3G8; RRID: AB_2800738 anti-Human CD19 (Mouse monoclonal) BioLegend Cat# 302265; Clone HIB19; RRID: AB_2800741 anti-Human CD45RO (Mous...
(cat. 555432), CD127 (cat. 560549), CD8 (cat. 347314), CD19 (cat. 340865), CD16 (cat. 338440) and CD14 (cat. 340660) antigens. The last 4 mAbs were conjugated with the same fluorochrome with the aim to cut-off in one step cytotoxic T cells (Tc), B cells, NK cells and ...
(HLA-DR−/lowCD11b+CD33+CD14−CD15+), CD11b+CD14−CD15+cells (alternative definition of G-MSCSs), HLA-DR−/lowCD14+cells (alternative definition of M-MSCSs), and HLA-DR−/lowCD33+CD16−cells in the peripheral blood from ten healthy donors before (homeostasis peripheral blood...
CD4 T cells were enriched by untouched magnetic cell separation (MACS) using the CD4 T cell isolation kit with additionally added CD14 beads for more profound monocyte depletion (Miltenyi Biotec, Bergisch Gladbach, Germany). To obtain memory CD4 T cells, naïve and effector cells were depleted ...
MDSCs can be further divided into two major subsets: the granulocytic MDSCs that display CD14−CD15+CD11b+CD33+ HLA-DRlow/− phenotype and monocytic MDSCs which have the phenotype of CD14+CD15−CD11b+CD33+ HLA-DR low/− (Damuzzo et al., 2015). The highly suppressive MDSCs ...