The binding of miR-383-5p and 3'UTR of CENPF mRNA was indicated by dual-luciferase assays. Results: We found that miR-383-5p inhibited the cells proliferation, migration and invasion, and promoted apoptosis of M14 and A375 cells. Biochemical analysis revealed that the expression of miR-383-...
dictive value of miR￣383￣5p and KIF3B mRNA expression on the occurrence of cSCC.Ｒｅｓｕｌｔｓ㊀Compared with the adjacent tissue,the expression of miR￣383￣5p in the cancer tissue was decreased,and the expression of KIF3B mRNA was increased (t=31.868,33.517,both P<0.001).Pearson ...
rabbits;preadipocytes;miR-383-5p;RAD51AP1;differentiation;proliferation 1. Introduction Obesity is now considered to be an epidemic, spreading rapidly not only in developed countries but also in developing countries [1,2]. Obesity has a positive and significant impact on the prevalence of all chron...
The study suggests that SHMT2 and miR-383-5p could be potential therapeutic targets for LUAD treatment. However, further research is needed to confirm these findings and explore their effects on lung cancer growth and metastasis. 展开 关键词: MICRORNA LIVER cancer CANCER cell proliferation CANCER...
目的探讨miR-140-5p调控FGF9在拇外翻中的作用,从基因层面阐明拇外翻的发病机制,为临床预防和治疗拇外翻提供基础理论依据.方法收集2013~2015年于抚顺市中心医院就诊的拇外翻患者(10例)和非拇外翻患者(5例)的第一跖骨内侧骨组织及表面软组织样本,运用Western blot以及real-time PCR检测各组织中miR-140-5p和FGF9的表...
【摘要】目的通过检测口腔癌皮下移植瘤小鼠小肠中代谢物和代谢通路的变化,分析过表达miR-181a-5p对皮下移植瘤小鼠小肠中代谢物和代谢物组的影响。方法实验分为3组,空白对照(Control)组、阴性对照(negative control,NC)组以及实验(over expression of miR-181a-5p,OE)组。将不同组别处理后的细胞混悬液,通过皮下...
miR-383-5pRAD51AP1differentiationproliferationObesity has become a major health problem worldwide, and increasing evidence supports the importance of microRNAs (miRNAs) in its pathogenesis. Recently, we found that miR-383-5p_1 is highly expressed in the perirenal fat of high-fat-fed rabbi...
增殖miRNA为了研究miR-383-5p对大鼠肝细胞凋亡和增殖的影响,试验用不同浓度的miR-383-5p模拟物(mimics)或抑制剂(inhibitor)对大鼠肝细胞进行不同时间的瞬时转染,利用qRT-PCR方法检测miR-383-5p的相对表达量,筛选在肝细胞中过表达和干扰表达miR-383-5p的适宜条件;利用流式细胞仪研究miR-383-5p过表达和干扰表达对...
Serum miR-574-5p: a prognostic predictor of sepsis patients. Serum micro-RNAs (miRNAs) can be used for the diagnosis and prognosis of various diseases. Using genome-wide scans, we sought to identify serum miRNAs that... H Wang,K Meng,W Chen,... - 《Shock》 被引量: 127发表: 2012年...
双荧光素酶报告实验验证MALAT1和mi R-383-5p的靶向关系.NB组织和细胞系(NMB,SHEP21N,SHEP2)中MALAT1表达上调,miR-383-5p表达下调,其中SHEP2细胞中MALAT1表达水平最高,miR-383-5p表达水平最低.敲低MALAT1表达或过表达mi R-383-5p可明显降低SHEP2细胞增殖活性,迁移细胞数目和侵袭细胞数目,并下调Cyclin D1,...