在大鼠左心房注射10 μL si-MIAT或si-NC(滴度为1×10 8 T),取各组大鼠心肌组织,按“2.1.4”项下方法检测miR-384-5p基因表达。 2.2.2沉默miR-384-5p基因表达逆转BCF的抗心肌缺血损伤作用动物随机分为shmiRNA-384-5p组、AMI+shmiRNA-384-5p组、AMI+BCF组和AMI+BCF+shmiRNA-384-5p组,每组5只。通过在...
miR-384-5pMPP+细胞凋亡氧化应激目的 探讨杭白菊总黄酮对1-甲基-4-苯基吡啶离子(MPP+)诱导的人神经母细胞瘤细胞(SK-N-SH细胞)凋亡和氧化应激的影响及可能机制.方法 利用2.5 mmol/LMPP+诱导SK-N-SH细胞建立细胞损伤模型,MPP+诱导SK-N-SH细胞的同时加入0.1,0.2,0.4 mg/mL杭白菊总黄酮或(和)转染miR-384-...
在大鼠左心房注射10 μL si-MIAT或si-NC(滴度为1×10 8 T),取各组大鼠心肌组织,按“2.1.4”项下方法检测miR-384-5p基因表达。 2.2.2沉默miR-384-5p基因表达逆转BCF的抗心肌缺血损伤作用动物随机分为shmiRNA-384-5p组、AMI+shmiRNA-384-5p组、AMI+BCF组和AMI+BCF+shmiRNA-384-5p组,每组5只。通过在...
cgr-miR-384-5p qPCR试剂盒 分类:microRNA qPCR Kits 品牌:BioTNT 货号:cgr-miR-384-5p 原价:￥4030 现价:￥3098.38 节省:￥932 规格:1 kits 说明:cgr-miR-384-5p 特异性茎环引物试剂盒 ￥615产品参数 ---说明书下载--- microRNAs(miRNAs)是一类长度约19~24nt的非编码单链RNA,通过碱基互补配对的方...
miR-384-5p体外靶向并抑制Lepr的表达.MDP干预骨细胞后提取的上清液促进BMSCs的成骨分化,抑制其成脂分化,同时调节BMSCs成骨分化过程中miR-384-5p和Lepr的表达.结论:(1)MDP可引起不同饮食喂养下的小鼠出现能量代谢和骨形成的改变,这与骨组织中LEPR表达改变相关.(2)MDP可通过骨细胞调控miR-384-5p/Lepr的表达促进...
3. LTP maintenance requires decreases in miR-26a and miR-384-5p. Since the effect size of miRNAs on LTP are likely to be positively correlated with their fold changes, we examined the roles of miR-384-5p (with the largest fold change and smallest P value) and miR-26a (with the second...
miR-384-5p overexpression or SIRT1 knockdown in the presence of circR-ZC3HC1 overexpression in OGD-treated neurons lead to reduced autophagy and enhanced apoptosis. Conclusion Collectively, circR-ZC3HC1 promoted neuronal autophagy to attenuate IS via miR-384-5p/SIRT1 axis....
cqvip:目的研究miR-384-5p是否通过调节肾小管上皮细胞BMP7转录而影响肾纤维化。方法建立小鼠单侧输尿管阻塞模型(unilateral ureteral obstructive,UUO),流式细胞仪荧光激活细胞分选(FACS)分离肾组织获取肾小管上皮细胞进行培养,通过脂质体转染表达miR-384-5p的质粒、表达反义miR-384-5p的质粒及...
Recently, the micro RNA, miR-384-5p, was reported to play a significant role in cell survival in response to different insults. Methods In vitro model of traumatic neuronal injury was induced by application of a sharp sterile blade to generate cuts in PC12 cells, and in vivo SCI was ...
Using next-generation sequencing to profile miRNA transcriptomes, we demonstrate that miR-26a and miR-384-5p specifically affect the maintenance, but not induction, of LTP and different stages of spine enlargement by regulating the expression of RSK3. Using bioinformatics, we also examine the global...