本发明证明了lifr是mir-196a-5p的靶向结合位点,mir-196a-5p过表达抑制lifr表达,mir-196a-5p低表达促进lifr表达。因此mir-196a-5p可以用于制备调控lifr表达的试剂。 技术特征: 1.mir-196a-5p在制备调控lifr表达的试剂中的应用。 2.如权利要求1所述的应用,其特征在于,mir-196a-5p过表达抑制lifr表达,mir-196...
MiR-196a-5pBACH1HypertensionVascular remodelingOxidative stressHyperproliferation of vascular smooth muscle cells (VSMCs) is a driver of hypertensive vascular remodeling. This study aimed to uncover the mechanism of BTB and CNC homology 1 (BACH1) and microRNAs (miRNAs) in VSMC growth and ...
目的: 探讨miR-196-5p对皮肤鳞状细胞癌(CSCC)增殖、侵袭和EMT的影响及其潜在的作用机制。方法:利用实时荧光定量PCR(RT-qPCR)检测miR-196-5p在癌旁正常组织、CSCC组织、A431细胞和HaCaT细胞中的表达水平。过表达或下调miR-196-5p在A431细胞中的表达后,通过CCK 8实验检测miR-196-5p对A431细胞增殖能力的影响;细胞...
临床参数相关分析显示 miR-196a-5p 低表达与较大的肿瘤体积(≥5cm,p=0.0135)及顺铂化疗耐药(p˂0.0001)显著相关。然后借助 ROC 曲线的曲线下面积(AUC)和 Kaplan-Meier Plotter 数据库发现 miR-196a-5p 可作为预测耐药(AUC=0.8552)和生...
[摘要] 目的探讨miR-196-5p对皮肤鳞状细胞癌(CSCC)增殖、侵袭和EMT的影响及其潜在的作用机制。方法利用实时荧光定量PCR(RT-qPCR)检测miR-196-5p在癌旁正常组织、CSCC组织、A431细胞和HaCaT细胞中的表达水平。过表达或下调miR-196-5p在A...
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The calculated Area under curve (AUC) levels showed that miR-196a-5p (AUC: 0.64; 95% CI: 0.521–0.759;=0.032) for obstructive CAD and STEMI groups, miR-373-3p (AUC: 708; 95% CI: 0.605–0.812;) for STEMI group and miR-375 (AUC: 0.722; 95% CI: 0.635–0.808;<) for obstructive...
miR-196a-5p was correlated with CAG progression to GC. Overexpression of miR-196a-5p promoted GC cell proliferation and migration and inhibited apoptosis, whereas suppression of miR-196a-5p exerted the opposite effect. Based on the prediction and luciferase assays, ACER2 was identified as the ...
In pull-down assays, there was a 3.4- and 5.2-fold increase in miR-196a-5p (P = 0.054) and miR-196b-5p (P = 0.035), respectively, using the UGT2A1 3'-UTR biotinylated mRNA probe as compared with the β-actin coding region control mRNA probe. UGT2A1 mRNA was reduced by 25% ...
www.nature.com/scientificreports OPEN Common SNP in hsa-miR-196a-2 increases hsa-miR-196a-5p expression and predisposes to received: 22 July 2015 idiopathic male infertility in Chineseaccepted:07December2015 Han populationPublished:25January2016 Jing Lu1, Hao Gu2,3, Qiuqin Tang4, Wei...