本发明提供了miR‑196a‑5p在制备调控LIFR表达的试剂中的应用,本发明属于生物医药技术领域。本发明证明了LIFR是miR‑196a‑5p的靶向结合位点,miR‑196a‑5p过表达抑制LIFR表达,miR‑196a‑5p低表达促进LIFR表达。因此miR‑196a‑5p可以用于制备调控LIFR表达的试剂。 技术研发人员:张俊河,梁帆,高建辉...
目的: 探讨miR-196-5p对皮肤鳞状细胞癌(CSCC)增殖、侵袭和EMT的影响及其潜在的作用机制。方法:利用实时荧光定量PCR(RT-qPCR)检测miR-196-5p在癌旁正常组织、CSCC组织、A431细胞和HaCaT细胞中的表达水平。过表达或下调miR-196-5p在A431细胞中的表达后,通过CCK 8实验检测miR-196-5p对A431细胞增殖能力的影响;细胞...
Part C 为指标的特异性引物试剂盒,试剂盒包括进行qPCR的上下游引物,以及逆转录使用的特异性茎环引物,该部分可以单独购买; 另试剂盒中提供一支RNA 酶DNA 酶free的双蒸水,可用于360次体系的配制。 本试剂盒目前订购,还可赠送内参的特异性引物试剂盒,该试剂盒包括检测该内参的qPCR上下游引物,以及逆转录使用的特异性...
Xin et al. BMC Cancer (2019) 19:30 https://doi.org/10.1186/s12885-018-5245-1 RESEARCH ARTICLE miR-196a-5p promotes metastasis of colorectal cancer via targeting IκBα He Xin, Chuanzhuo Wang and Zhaoyu Liu* Open Access Abstract Background: MicroRNA-196a-5p (miR-196a-5p) has been ...
Hong等[12]研究发现,与健康对照组相比,肾病患者中miR-193a-3p表达升高,miR-193a-3p参与了肾病的发病机制和疾病进展,可为肾病诊断提供重要的帮助。Hu等[13]研究表明,miR-196-5p在细胞生物合成、代谢调控过程中发挥重要作用,可通过调节肾...
miR-196a-5p was correlated with CAG progression to GC. Overexpression of miR-196a-5p promoted GC cell proliferation and migration and inhibited apoptosis, whereas suppression of miR-196a-5p exerted the opposite effect. Based on the prediction and luciferase assays, ACER2 was identified as the ...
The miR-196a-5p- and miR-196b-55p-treated cells exhibited reductions of 35% (P = 0.047) and 44% (P = 0.0063), respectively. These data suggest that miR-196a-5p and miR-196b-5p play an important role in UGT2A1 regulation within the lung and potentially other aerodigestive tract ...
miR-196b-5p上游引物序列为5’-GGGTAGGTAGTTTCCTG-3’,下游引物序列为5’-AACTGGTGTCG TGGAGTCGGC-3’;内参基因U6上游引物序列为5’-CTCGCTTCGGCAGCACATATACT-3’,下游引物序列为5’-ACGCTTCACGAATTTGCGT GTC-3’。PCR扩增...
Results: miR-196-5p and miR-361-5p were, respectively, up- and down-regulated in COPD patients compared with healthy controls. Luciferase assays demonstrated that miR-361-5p and miR-196-5p were, respectively, targeting abca1 and arhgef12 3ʹUTR in BEAS-2B cells. GS significantly ...