本发明证明了lifr是mir-196a-5p的靶向结合位点,mir-196a-5p过表达抑制lifr表达,mir-196a-5p低表达促进lifr表达。因此mir-196a-5p可以用于制备调控lifr表达的试剂。 技术特征: 1.mir-196a-5p在制备调控lifr表达的试剂中的应用。 2.如权利要求1所述的应用,其特征在于,mir-196a-5p过表达抑制lifr表达,mir-196...
hsa-miR-196a-5p inhibitor CAS No. : MCE 国际站:hsa-miR-196a-5p inhibitor 产品活性:hsa-miR-196a-5p inhibitor 是一种全链经过甲氧基修饰的成熟miRNA的互补单链。miRNA inhibitor通过特异性结合成熟miRNA,阻止miRNA与其靶基因的互补配对,从而抑制miRNA的功能,可用于miRNA 功能缺失性(loss-of-function)研究...
hsa-mir-196a-5p Real-time RT-PCR Detection Kit 中文名: 中文别名: CBNumber: CB55996439 分子式: 分子量: 0 MOL File: Mol file 化学性质安全信息用途供应商1 hsa-mir-196a-5p Real-time RT-PCR Detection Kit化学性质 安全信息 hsa-mir-196a-5p Real-time RT-PCR Detection Kit性质、用途与生产工艺...
Part C 为指标的特异性引物试剂盒,试剂盒包括进行qPCR的上下游引物,以及逆转录使用的特异性茎环引物,该部分可以单独购买; 另试剂盒中提供一支RNA 酶DNA 酶free的双蒸水,可用于360次体系的配制。 本试剂盒目前订购,还可赠送内参的特异性引物试剂盒,该试剂盒包括检测该内参的qPCR上下游引物,以及逆转录使用的特异性...
miRNA为miR-196a-5p,miR-105-5p.纳入良性肺结节组患者26例,恶性肺结节组患者46例.良,恶性结节组血清miR-196a-5p水平[M(P25,P75)]分别为0.63(0.09,2.15),1.93(0.93,4.97),miR-105-5p水平分别为2.03(0.54,7.95),10.65(5.94,18.39).与良性肺结节组相比,恶性肺结节组血清miR-196a-5p,miR-105-5p水平升高...
miR-196a-5p was correlated with CAG progression to GC. Overexpression of miR-196a-5p promoted GC cell proliferation and migration and inhibited apoptosis, whereas suppression of miR-196a-5p exerted the opposite effect. Based on the prediction and luciferase assays, ACER2 was identified as the ...
In pull-down assays, there was a 3.4- and 5.2-fold increase in miR-196a-5p (P = 0.054) and miR-196b-5p (P = 0.035), respectively, using the UGT2A1 3'-UTR biotinylated mRNA probe as compared with the β-actin coding region control mRNA probe. UGT2A1 mRNA was reduced by 25% ...
www.nature.com/scientificreports OPEN Common SNP in hsa-miR-196a-2 increases hsa-miR-196a-5p expression and predisposes to received: 22 July 2015 idiopathic male infertility in Chineseaccepted:07December2015 Han populationPublished:25January2016 Jing Lu1, Hao Gu2,3, Qiuqin Tang4, Wei...
Significant reductions in firefly luciferase activity were observed in human embryonic kidney cell line 293 cells cotransfected with the wild-type UGT2A1 3'-untranslated region (UTR)-containing luciferase plasmid and either miR-196a-5p (62%, = 0.0063), respectively. These data suggest that miR-...