作者们进一步通过肽-MHC稳定性测定(cell-based stability assay)和竞争结合测定(IC50 competition binding assay)的方法对有代表性的HLA等位基因进行了实验验证,包括 8种不同的HLA等位基因(136个肽-HLA组合)。 为了进一步对锚定位点的影响进行...
作者们进一步通过肽-MHC稳定性测定(cell-based stability assay)和竞争结合测定(IC50 competition binding assay)的方法对有代表性的HLA等位基因进行了实验验证,包括 8种不同的HLA等位基因(136个肽-HLA组合)。 为了进一步对锚定位点的影响进行分析,作者们针对923个选定的TCGA患者-HLA等位基因配对预测了新抗原。结果表明...
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LAG3/MHC II binding assay kit 英文别名: LAG3/MHC II binding assay kit 中文名: 中文别名: CBNumber: CB19717108 分子式: 分子量: 0 MOL File: Mol file 化学性质安全信息用途供应商1 LAG3/MHC II binding assay kit化学性质 安全信息 LAG3/MHC II binding assay kit性质、用途与生产工艺 ...
PBMC from LTBI donors were incubated with 10 mg/ml peptides from ESAT-6, and the number of IFN-g-producing cells was enumerated in an ELISPOT assay. ESAT-6 has four antigenic regions, indicated by capped lines. The numbers of donors tested for each region were n = 7 for Regions 1 ...
Figure 1.Plate Map of Peptide Binding Assay.(A) Map of the peptide dilutions in polypropylene 384-well plates. Each polypropylene plate can accommodate three groups of 15 peptides in competition binding reactions against a single MHC II allele. (B) After the binding reaction approaches equilibriu...
首先,作者开发了全新的肿瘤抗原预测算法hmMHC(a hidden Markov model-based MHC binding predictor),筛选和预测出mITGB1(integrin-β1)的N710Y突变体很可能是T3肉瘤细胞(T3 sarcoma cells)的MHC II型肿瘤抗原。接下来,作者通过ELISPOT(Enzyme-linked immune absorbent spot )等方法,证实了这一推测。
As assay for the measurement of direct binding of a peptide that is a T-cell epitope to gene products of the major histocompatibility complex (MHC), classes I and II, on the surface of intact living antigen-presenting cells, is provided. The assay comprises incubating the labelled peptide ...
首先,作者开发了全新的肿瘤抗原预测算法hmMHC(a hidden Markov model-based MHC binding predictor),筛选和预测出mITGB1(integrin-β1)的N710Y突变体很可能是T3肉瘤细胞(T3 sarcoma cells)的MHC II型肿瘤抗原。接下来,作者通过ELISPOT(E...
For HLA class II all CD4+ epitopes measured by Intracellular Cytokine Staining(ICS) assay were downloaded from the IEDB. The set was filtered to include only positive epitopes with four letter resolution HLA typing. Further, epitopes overlapping with the HLA-II training data (100% identity) were...