MDCK-MDR1 permeability assay: Cyprotex’s MDCK-MDR1 cells originate from the transfection of Madin Darby Canine Kidney (MDCK) cells with the human MDR1 gene (ABCB1)3, the gene encoding for the efflux transporter, human P-gp. Assessing flux in both directions (apical to basolateral (A-B)...
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One Assay, Two Vital Values‐Estimating Intrinsic Permeability and Efflux Using MDCK‐MDR1 Assaydoi:10.1096/fasebj.2022.36.S1.R2681Zang, GraceLi, ChunFASEB Journal
(P—gP)expression asmodels couldbeusedasa modeltoscreensubstratesor well cells,it drugtransport P-gP inhibitors,as ofintestinal brainbarrierand therearesome inMDCK- mucosa,blood kidney.Although disadvantages MDRlcell isaneffectivetoolfor of inan fashion. model,it implementingpermeabilitydrug optimal ...
where PappB-A and PappA-B are the mean permeability coefficients obtained for transport in the basolateral to apical (B-A) direction and the apical to basolateral (A-B) direction, respectively. ATP assay For the ATP assay, MDCKII-WT, MDCKII-MDR1 and Caco-2 cells seeded in 96-well plat...
Calcein-AM assay was found to be non-predictive to surfactants influence on digoxin permeability across cell monolayers. In conclusion, these results may assist, in a mechanism-based, selection of suitable surfactants for formulating oral dosage forms to enhance the absorption of low bioavailable P-...
Despite its known effect on digoxin pharmacokinetics [91,92], ritonavir’s low permeability prevents studying its interaction with MDR1 in vectorial transport assays [93]. However, ritonavir DDI potential was shown in another assay [94]. To reveal the IC50’s dependence on substrate, transporter...
1. Advances in the MDCK-MDR1 cell model and its applications to screen drug permeability; MDCK-MDR1细胞模型及其在药物透过研究中的应用进展2) MDCK-MDR1 cells MDCK MDR1细胞3) MDCK cell MDCK细胞 1. Effect of influenza virus on the expression of MDCK cellular nucler transcription factors in...