关键词:MDCK-MDR1细胞;P-糖蛋白;药物转运;细胞模型中图分类号 :R965.1 文献标识码:A 文章编号:0513-4870(2008)06-0559-06收稿日期:2007-11-15.基金项目:浙江省科技厅重大项目重点资助项目(2005C13026).* 通讯作者 Tel/ Fax:86-571 -88208407,E-mail:zengsu@zju.edu.cnAdvancesintheMDCK-MDR1 cellmodel...
Keywords:MDCK—MDR1cell;P—glycoprotein;drugtransport;cellmodel 膜转运蛋白对药物的吸收,排泄及代谢,起着 至关重要的作用.它可以保护细胞免受外来毒性物 质侵害.将哺乳动物肿瘤细胞暴露在一种细胞毒类 药物中一段时间后,这些细胞可通过降低胞内药物 浓度而对一系列的药物产生抗性.原因主要是多药 耐药基因(muhidr...
Keywords:MDCK—MDR1cell;P—glycoprotein;drugtransport;cellmod el 膜转运蛋白对药物的吸收,排泄及代谢,起着 至关重要的作用.它可以保护细胞免受外来毒性物 质侵害.将哺乳动物肿瘤细胞暴露在一种细胞毒类 药物中一段时间后,这些细胞可通过降低胞内药物
of multiple substrates: influenceofcell andmodulatorconcentrationon 尽管如此,MDCK.MDRl细胞用途广、作用大、 line P—gP Metab activity[J].JDrug 实验周期短,是很有前景的实验模型,需要在今后的 1687. 实验中进一步完善和发展。 a1.Evaluationof JD,WeinsteinK,et [15]WangQ,Rager forthe theMDR—MDCKcell...
关键词: MDCK—MDR l细胞; P 一糖蛋白; 药物转运; 细胞模型中图分类号: R965. 1文献标识码: A文章编号: 0513—4870(2008)06—0559—06AdvancesintheMDCK-MDRlcellmodelanditsapplications-toscreendrugDermeability…■・L IUYao,ZENGSu’(CollegeofPharmaceuticalSciences,ZhejiangUniversity,Hangzhou310058,China)...
MDCK–MDR1 cellspermeabilityquantum dotnanoparticleAim: To investigate the function of CdSe/ZnS and CdTe/ZnTe nanoparticles through Caco-2,MDCK and MDCK-MDR1 cell monolayers.Methods: Using enterocyte-like Caco-2 cells as a small intestine epithelial model,Madin鈥...
This article introduces the characteristics and establishment of MDCK-MDR1 cell line, and its applications as an model for the assessment of the membrane p... 曾苏 - 《药学学报》 被引量: 0发表: 0年 防己诺林碱调控紫杉醇在MDR1-MDCKⅡ细胞模型中的跨膜转运研究 目的:研究防己诺林碱对紫杉醇在MDR...
This article introduces the characteristics and establishment of MDCK-MDR1 cell line, and its applications as an model for the assessment of the membrane p... 曾苏 - 《药学学报》 被引量: 0发表: 0年 防己诺林碱调控紫杉醇在MDR1-MDCKⅡ细胞模型中的跨膜转运研究 目的:研究防己诺林碱对紫杉醇在MDR...
The review summarizes the progress and applications of MDCK-MDR1 cell model. Because there is high polarized P-glycoprotein (P-gp) expression in MDCK-MDR1 cells, it could be used as a drug transport model to screen P-gp substrates or inhibitors, as well as models of intestinal mucosa, ...
Faster cell growing time Lower TEER values Can be used as anin vitro blood-brain barrier (BBB) model Brief Protocol Similar to the Caco-2 assay, the MDR1-MDCK assay also detects the permeation efficiency of compounds from apical to basolateral (A→B), or from basolateral to apical (B→A...