MDCK-MDR1细胞药物体外吸收模型的建立及验证
This method uses the MDR1-MDCK cell line, a combination of Madin Darby Canine Kidney (MDCK) cells, and the MDR1 gene to encode the efflux protein, P-gp. The MDR1-MDCK permeability assay is a valuable tool to identify and characterize P-gp substrates and inhibitors, and it can help ...
(brainmicrovascularendothelialcells,BMECs)上 P—gP 的外排作用实现的.BBB 的体外模型BMECs 与I 型星形胶质细胞(typeIastrocytes,TIA)体外共 培养易丢失部分甚至全部的生理学特性,并且生存 时间短,传代次数少.而MDCK—MDR1 细胞是作为 BBB 药物透过快速筛选的理想模型¨.Madgula 等以MDCK—MDR1 细胞为...
英文名称MDR1 transfection into canine kidney cells MDCK-MDR1 英文同义词MDR1 transfection into canine kidney cells MDCK-MDR1 CAS号 分子式 分子量0 EINECS号 相关类别 Mol文件Mol File 结构式 MDR1转染犬肾细胞MDCK-MDR1 性质 MDR1转染犬肾细胞MDCK-MDR1 用途与合成方法 ...
Human MDR1 Vesicles are part of a larger group of transporter vesicles known as ABC transporter vesicles. They are prepared from purified plasma membranes isolated from an insect cell system (Sf9 cells transfected with baculovirus) expressing MDR1. MDR1 is an important determinant of drug dispositi...
有报道∞引,经转染了人 的稳定,这一作用部分是通过脑微血管内皮细胞 mdrl基因的MDCK细胞内的神经酰胺己三糖苷含 microvascularendothelial (brain cells,BMECs)上 P-gp的外排作用实现的。BBB的体外模型BMECs体,从而使得MDCK—MDRl细胞对细菌外毒素的敏 I astrocytes,TIA)体外共感性增加了1×105—1×106倍。 与I...
(2012). MDR-1-overexpression in HT 29 colon cancer cells grown in SCID mice. Acta Histochem 114, 594-602.Schumacher U, Nehmann N, Adam E, Mukthar D, Slotki IN, Horny HP, et al: MDR‑1‑overexpression in HT 29 colon cancer cells grown in SCID mice. Acta Histochem 114: ...
克隆类型:Poolcells 种属来源:犬 组织来源:犬肾 疾病特征:—— 细胞形态:上皮细胞样 生长特性:贴壁生长 培养体系:DMEM-H+10% FBS+1% P/S 配套培养基货号:TCO-G043 传代比例:1:2-1:3,每2-3天换液一次 传代周期:24-48 h 培养条件:气相:95%空气+5%CO2,温度:37℃ 冻存条件:60%基础培养基+30%FBS+...
cells were used to determine the passive permeability of ML120B and its permeation mechanism. Results The passive permeability of ML120B was 678.9 nm·s -1 and the efflux ratio was 0.8 without Pgp inhibitor and 0.5 with Pgp inhibitor. Conclusions ML120B is a highly permeable compound and is...
Human MDR1 Vesicles are part of a larger group of transporter vesicles known as ABC transporter vesicles. They are prepared from purified plasma membranes isolated from an insect cell system (Sf9 cells transfected with baculovirus) expressing MDR1. MDR1 is an important determinant of drug dispositi...