Using MDCK-MDR1 cells (a transfected cell line overexpressing human P-glycoprotein (multidrug resistance 1; MDR1)) to evaluate whether your compound is a substrate of the P-glycoprotein (P-gp) efflux transporter is crucial in early drug discovery/development as such knowledge feeds into both: ...
MDCK-MDR1细胞药物体外吸收模型的建立及验证
This method uses the MDR1-MDCK cell line, a combination of Madin Darby Canine Kidney (MDCK) cells, and the MDR1 gene to encode the efflux protein, P-gp. The MDR1-MDCK permeability assay is a valuable tool to identify and characterize P-gp substrates and inhibitors, and it can help ...
有报道∞引,经转染了人 的稳定,这一作用部分是通过脑微血管内皮细胞 mdrl基因的MDCK细胞内的神经酰胺己三糖苷含 microvascularendothelial (brain cells,BMECs)上 P-gp的外排作用实现的。BBB的体外模型BMECs体,从而使得MDCK—MDRl细胞对细菌外毒素的敏 I astrocytes,TIA)体外共感性增加了1×105—1×106倍。 与I...
(brainmicrovascularendothelialcells,BMECs)上 P—gP 的外排作用实现的.BBB 的体外模型BMECs 与I 型星形胶质细胞(typeIastrocytes,TIA)体外共 培养易丢失部分甚至全部的生理学特性,并且生存 时间短,传代次数少.而MDCK—MDR1 细胞是作为 BBB 药物透过快速筛选的理想模型¨.Madgula 等以MDCK—MDR1 细胞为...
(P—gP)expressioninMDCK—MDRlcells,itcouldbeusedasadrugtransportmodeltoscreenP-gPsubstratesorinhibitors,aswellasmodelsofintestinalmucosa,bloodbrainbarrierandkidney.AlthoughtherearesomedisadvantagesinMDCK-MDRlcellmodel,itisaneffectivetoolforimplementingpermeabilityofdruginanoptimalfashion.Keywords:MDCK-MDRlcell;P—...
英文名称MDR1 transfection into canine kidney cells MDCK-MDR1 英文同义词MDR1 transfection into canine kidney cells MDCK-MDR1 CAS号 分子式 分子量0 EINECS号 相关类别 Mol文件Mol File 结构式 MDR1转染犬肾细胞MDCK-MDR1 性质 MDR1转染犬肾细胞MDCK-MDR1 用途与合成方法 ...
CAS号: 英文名: MDR1 transfection into canine kidney cells MDCK-MDR1 英文别名: MDR1 transfection into canine kidney cells MDCK-MDR1 中文名: MDR1转染犬肾细胞MDCK-MDR1 中文别名: MDR1转染犬肾细胞MDCK-MDR1 CBNumber: CB48002572 分子式:
cells were used to determine the passive permeability of ML120B and its permeation mechanism. Results The passive permeability of ML120B was 678.9 nm·s -1 and the efflux ratio was 0.8 without Pgp inhibitor and 0.5 with Pgp inhibitor. Conclusions ML120B is a highly permeable compound and is...
Influence of borneol and muscone on geniposide transport through MDCK and MDCK-MDR1 cells as blood-brain barrier in vitro model. Int. J. Pharm. 2013, 456, 73-79. [CrossRef] [PubMed]Chen, Z.; Lu, Y.; Du, S.; Shang, K.; Cai, C. Influence of borneol and muscone on geniposide ...