(P-gp)expressioninMDCK-MDR1cells, itcouldbeusedasadrugtransportmodeltoscreenP-gpsubstratesorinhibitors, aswellasmodelsofintestinalmucosa, bloodbrainbarrierandkidney.AlthoughtherearesomedisadvantagesinMDCK-MDR1 cellmodel, itisaneffectivetoolforimplementingpermeabilityofdruginanoptimalfashion.Keywords:MDCK-MDR1 ...
MDCK-MDR1细胞药物体外吸收模型的建立及验证
cells,itcouldbeusedasadrugtransportmodeltoscreenP—gpsubstratesorinh ibitors,aswellasmodels ofintestinalmucosa,bloodbrainbarrierandkidney.Althoughtherearesomed isadvantagesinMDCK— MDR1cellmodel,itisaneffectivetoolforimplementingpermeabilityofdrug inanoptimalfashion. ...
有报道∞引,经转染了人 的稳定,这一作用部分是通过脑微血管内皮细胞 mdrl基因的MDCK细胞内的神经酰胺己三糖苷含 microvascularendothelial (brain cells,BMECs)上 P-gp的外排作用实现的。BBB的体外模型BMECs体,从而使得MDCK—MDRl细胞对细菌外毒素的敏 I astrocytes,TIA)体外共感性增加了1×105—1×106倍。 与I...
(brainmicrovascularendothelialcells,BMECs)上 P—gP 的外排作用实现的.BBB 的体外模型BMECs 与I 型星形胶质细胞(typeIastrocytes,TIA)体外共 培养易丢失部分甚至全部的生理学特性,并且生存 时间短,传代次数少.而MDCK—MDR1 细胞是作为 BBB 药物透过快速筛选的理想模型¨.Madgula 等以MDCK—MDR1 细胞为...
CAS号: 英文名: MDR1 transfection into canine kidney cells MDCK-MDR1 英文别名: MDR1 transfection into canine kidney cells MDCK-MDR1 中文名: MDR1转染犬肾细胞MDCK-MDR1 中文别名: MDR1转染犬肾细胞MDCK-MDR1 CBNumber: CB48002572 分子式:
(P—gP)expressioninMDCK—MDRlcells,itcouldbeusedasadrugtransportmodeltoscreenP-gPsubstratesorinhibitors,aswellasmodelsofintestinalmucosa,bloodbrainbarrierandkidney.AlthoughtherearesomedisadvantagesinMDCK-MDRlcellmodel,itisaneffectivetoolforimplementingpermeabilityofdruginanoptimalfashion.Keywords:MDCK-MDRlcell;P—...
Bergapten could reduce the accumulation of Rh123 in MDCK-MDR1 cells, increase the membrane fluidity, and upregulate P-gp protein and mRNA expression but it had no effect on P-gp ATPase activity.Conclusions: Overall, we concluded that the possible mechanism through which bergapten inhibits ...
(MDCK) cells, and the MDR1 gene to encode the efflux protein, P-gp. The MDR1-MDCK permeability assay is a valuable tool to identify and characterize P-gp substrates and inhibitors, and it can help understand the mechanism of drug efflux and highlight early potential issues with drug ...
cells/100mmdish/150cm 2 flask),冰上操作;收集裂解液至离心管中; B、在振荡器上混匀4~15min,14000g离心15min(4°C),弃沉淀; C、用Bradford法或其它蛋白质测定方法测定上清中蛋白浓度以调整上样体积和上样量; D、超声10~15秒剪切DNA以减低样品粘性; E、样品5minutes; F、离心12000g,5min,取上清; G...