关键词:MDCK-MDR1细胞;P-糖蛋白;药物转运;细胞模型中图分类号 :R965.1 文献标识码:A 文章编号:0513-4870(2008)06-0559-06收稿日期:2007-11-15.基金项目:浙江省科技厅重大项目重点资助项目(2005C13026).* 通讯作者 Tel/ Fax:86-571 -88208407,E-mail:zengsu@zju.edu.cnAdvancesintheMDCK-MDR1 cellmodel...
Keywords:MDCK—MDR1cell;P—glycoprotein;drugtransport;cellmodel 膜转运蛋白对药物的吸收,排泄及代谢,起着 至关重要的作用.它可以保护细胞免受外来毒性物 质侵害.将哺乳动物肿瘤细胞暴露在一种细胞毒类 药物中一段时间后,这些细胞可通过降低胞内药物 浓度而对一系列的药物产生抗性.原因主要是多药 耐药基因(muhidr...
Keywords:cellmodel,P-gp,Fluorescentyellowlabeling,two-waytransport 目录 TOC\o\h\z摘要 I ABSTRACT II 1前言 4 1.1MDCK细胞的来源及用途 4 1.2MDCK细胞培养方法及影响因素 4 1.3MDCK-MDR1模型的研究现状 4 1.3.1MDCK-MDR1模型的建立 4 1.3.2作为药物吸收实验的体外模型 5 ...
Liu, Y.; Zeng, S. Advances in the MDCK-MDR1 cell model and its applications to screen drug permeability. Yaoxue Xuebao 2008, 43, 559-64.Liu Y Zeng S.Advances in the MDCK-MDR1 cell model and its applications to screen drug permeability .药学学报,2008,:43-564,559...
关键词: MDCK—MDR l细胞; P 一糖蛋白; 药物转运; 细胞模型中图分类号: R965. 1文献标识码: A文章编号: 0513—4870(2008)06—0559—06AdvancesintheMDCK-MDRlcellmodelanditsapplications-toscreendrugDermeability…■・L IUYao,ZENGSu’(CollegeofPharmaceuticalSciences,ZhejiangUniversity,Hangzhou310058,China)...
Keywords:MDCK—MDR1cell;P—glycoprotein;drugtransport;cellmod el 膜转运蛋白对药物的吸收,排泄及代谢,起着 至关重要的作用.它可以保护细胞免受外来毒性物 质侵害.将哺乳动物肿瘤细胞暴露在一种细胞毒类 药物中一段时间后,这些细胞可通过降低胞内药物
of multiple substrates: influenceofcell andmodulatorconcentrationon 尽管如此,MDCK.MDRl细胞用途广、作用大、 line P—gP Metab activity[J].JDrug 实验周期短,是很有前景的实验模型,需要在今后的 1687. 实验中进一步完善和发展。 a1.Evaluationof JD,WeinsteinK,et [15]WangQ,Rager forthe theMDR—MDCKcell...
researchers. The use of cell culture provides a method for predicting drug permeability by using the cell monolayer in the dual-chamber diffusion system to simulate the process of drug entering the blood from the intestinal lumen. Among them, the Caco-2 model and MDR1-MDCK model are widely ...
MDCK-MDR1 permeabilityEfflux transporters facilitate the export of compounds from the cell, both in some cellular assays, also in ADME studies. These transporters belong to the ATP-binding cassette …
—细胞系高表达惶堑鞍甮琍—襊.呈极性分布,因而它可以作为药物转运模PgPMDCKMDRlP文章编号:———YaoZENG’200843(6)559564R9651A,,:—lineanddrugsscreeningsand甌—瓸cellsit,mucosablood.modelitfashion篗cellPglycoproteindrugtransportcell收稿期:—一.基金项目:浙江省科技厅重大项目重点资助项目.TelFax...