MDCK-MDR1 permeability assay: Cyprotex’s MDCK-MDR1 cells originate from the transfection of Madin Darby Canine Kidney (MDCK) cells with the human MDR1 gene (ABCB1)3, the gene encoding for the efflux transporter, human P-gp. Assessing flux in both directions (apical to basolateral (A-B)...
Creative Bioarrayis a reliable MDR1-MDCK permeability assay provider. After years of development,Creative Bioarrayhas become an expert in drug discovery and development. Using a highly automated approach, we offer this in vitro ADME screening service to determine drug permeability. ...
ICE Bioscience generated MDCKII-MDR1 cell line in house by lentiviral system in-house, also developed permeability assay in both parental MDCKII and MDCKII-MDR1 cells. As there is some basic transportation activity in wildtype cell line, currently we are constructing MDCKII-KO-Pgp cell line ...
Additionally, the utility of MDCK-MDR1 permeability measurements was demonstrated in probing the role of P-glycoprotein transport in Primaquine鈥揅hloroquine drug鈥揹rug interactions in comparison with in vivo pharmacokinetic changes. This study provides an in-depth comparison of the Caco-2 and MDCK...
Permeability data from MDR1-MDCKII and PAMPA-BBB assays were compared to data from in situ brain perfusion to evaluate the accuracy of in vitro assays in predicting in vivo blood–brain barrier (BBB) permeability. PAMPA-BBB significantly correlated to in situ brain perfusion, however, MDR1-MDCK...
Creative Biolabs provides reliable MDCK and MDR1-MDCK permeability assay services to determine drug permeability
■· applications toscreen drugDermeability LIU Su’ Yao,ZENG 310058,China) (CollegeofPharmaceuticalSciences,ZhejiangUniversity,Hangzhou articleintroducesthecharacteristicsandestablishmentofMDCK—MDRleell Abstract:This line。and its asaninvitromodelfortheassessmentofthemembrane ofdrugs. applications permeability...
The MDCKII-LE apparent permeability values well differentiates compounds from high to medium/low human intestinal absorption and can be used for Biopharmaceutical Classification System. The MDCKII-LE permeability assay (4-in-1 cassette dosing) is high throughput with good precision, reproducibility, ...
1. Methods The inhibitory cytopathic effects (CPE) of catechu extract on influenza A virus were observed by MDCK cell culture techniques. 方法 用狗肾传代MDCK细胞培养法观察儿茶提取物抑制甲型流感病毒的致细胞病变作用;用鸡胚培养法观察儿茶提取物抑制甲型流感病毒增生的作用;用红细胞凝集试验观察儿茶提取物...
Verapamil displayed a vectorial basolateral-to-apical transepithelial efflux across the MDCK II MDR1 monolayers with a permeability of 5.7×105cmsec1 compared to an apical to basolateral permeability of 1.3×105cmsec-1. The efflux could be inhibited with the P-gp inhibitor zosuquidar. Zosuquid...