m6A-binding proteinsN6-methyladenosine (m6A) is a well-known post-transcriptional modification that is the most common type of methylation in eukaryotic mRNAs. The regulation of m6A is dynamic and reversible, which is erected by m6A methyltransferases ("writers") and removed by m6A demethylases (...
Diverse RNAs and RNA-binding proteins form phase-separated, membraneless granules in cells under stress conditions. However, the role of the prevalent mRNA methylation, m6A, and its binding proteins in stress granule (SG) assembly remain unclear. Here, we show that m6A-modified mRNAs are enriched...
It is known that m6A modification can recruit particular “reader” proteins or alter the structure of mRNA to modulate the processing, stability, and translation of mRNA [32,33]. Among them, YTHDF2, the binding protein of m6A, was the first discovered and most efficient m6A “reader” [29...
Interestingly, one of the most highly enriched m6A-binding proteins that we identified was RNA binding motif-containing protein 45 (RBM45), a protein that is abundant in the developing brain and that has been implicated in neurodegenerative disease (Tamada et al., 2002; Collins et al., 2012;...
Genome Biology (2023) 24:103 https://doi.org/10.1186/s13059-023-02947-4 Genome Biology RESEARCH Open Access m6A readers ECT2/ECT3/ECT4 enhance mRNA stability through direct recruitment of the poly(A) binding proteins in Arabidopsis Peizhe Song1†, Lianhuan Wei1†, Zixin ...
RNA structure constitutes a new layer of gene regulatory mechanisms. RNA binding proteins can modulate RNA secondary structures, thus participating in post-transcriptional regulation. The DEAH-box helicase 36 (DHX36) is known to bind and unwind RNA G-qua
Therefore, identifying the YTH proteins is crucial for unraveling the mechanisms underlying the function of m6A in cells. The YTH domain ranging in length from 100 to 150 amino acids, is rich in aromatic residues and can bind to single-stranded RNA sequences. This domain, which belongs to ...
RNA-binding proteinsCARCINOGENESISFORMALDEHYDEMACROPHAGESGENE expressionHISTOLOGICAL techniquesCANCER genesBackground: Dysregulation of RNA N6-methyladenosine (m6A) modification is indispensable in tumorigenesis. However, in muscle-invasive bladder cancer (MIBC), the key regulators and mechani...
Specific recognition of N6 -methyladenosine (m 6 A) in mRNA by RNA-binding proteins containing a YT521-B homology (YTH) domain is important in eukaryotic gene regulation. The Arabidopsis YTH-domain protein ECT2 is thought to bind to mRNA at URU(m 6 A)Y sites, yet RR(m 6 A)CH is ...
RNA-binding proteinsRNA methylationRNAMOLECULESCATALYTIC activitySimple Summary: Interest in RNA methylation has surged in recent years with the identification of the major eukaryotic mRNA m6A methyltransferases, METTL3 and METTL16. Interestingly, both METTL3 and METTL16 have "methylat...