Cell (2019):. 202003DOTBLOT 3.IP(+143) N6-methyladenineinDNAantagonizesSATB1inearlydevelopment.LiZ,ZhaoS,NelakantiRV,LinK,WuTP,AldermanMH,GuoC,WangP,ZhangM,MinW,JiangZ,etal. Nature (2020):. 202003IP;testedspecies:mouseDecipheringthe"m6ACode"viaAntibody-IndependentQuantitativeProfiling.Garcia-Campos...
m6A antibodyMeRIP SeqHuman fetal brainHuman fetal liverMouse liverMouse brainThe most effective method for mapping N6-methyladenosine (m6A) is m6A RNA immunoprecipitation sequencing (MeRIP-seq). The quality of MeRIP-seq relies on various factors, with the anti-m6A antibody being a crucial ...
Transcriptome-wide mapping of m6A modification in different cell types using methylated RNA immunoprecipitation and sequencing (meRIP-seq), as well as more recently using antibody-independent approaches, has revealed that m6A occurs on large subsets of several thousand mRNAs within a particular cell, an...
These results suggest the specificity of the m6A antibody (Figure S2H). Mettl3 regulates the proliferation and differentiation of aNSCs We next aimed to study the regulatory roles of Mettl3 in the proliferation and differentiation of aNSCs. We adopted a lentivirus to deliver a short hairpin RNA...
leukemia cells [38,63]. Previous studies indicated that TNFR2 binds to its ligand TNF, to mediate the binding of some adaptor proteins, which in turn initiate signal transduction to regulate cell death [80]. Thus, YTHDF2 may take part in inhibiting cell apoptosis by TNF signaling (Fig.3B...
A volume of 10ul sterile PBS contained 20ug Mouse CXCR2/IL-8RB Antibody (R&D, MAB2164) was administered into the femur through the kneecap using a 30–gauge insulin needle, followed by intraperitoneal injection with LPS after 2h. Cell population of bone marrow and blood was collected for ...
Cell lysates were immunoprecipitated using Flag-M2 beads and western blot for total or lactylation of METTL16 using anti-Flag or METTL16K229-lac antibody. d Flag-METTL16 and HA-SIRT2 plasmids were co-transfected into HGC27 cells followed by immunoprecipitation. e The Flag-METTL16 plasmid was...
6-甲基腺嘌呤(N6-methyladenosine,m6A)——即RNA中腺苷酸(A)第六位N上发生的甲基化,是真核生物mRNA中最丰富的表观转录组学修饰,占所有腺苷的0.1%-0.4%左右 [1] …
Methylation of nucleotides, notably in the forms of 5-methylcytosine (5mC) in DNA and N6-methyladenosine (m6A) in mRNA, carries important information for gene regulation. 5mC has been elucidated to participate in the regulation of fruit ripening, whereas
We present m6ACali, a novel machine-learning framework aimed at enhancing the accuracy of N6-methyladenosine (m6A) epitranscriptome profiling by reducing the impact of non-specific antibody enrichment in MeRIP-Seq. The calibration model ... H Ye,T Li,DJ Rigden,... - 《Nucleic Acids Research》...