lysosomal thiol proteaseHydrolytic activity of cathepsins B, H and trypsimlike proteases was measured in 38 serous middle ear effusion (MEE) samples. The conc:entrations of al1 antitrypsin (al1-AT) and al2-macroglobulin (al2-M) were also quantitated. The mean value of cathepsin B activity...
Variants in theCTSBgene encoding the lysosomal hydrolase cathepsin B (catB) are associated with increased risk of Parkinson’s disease (PD). However, neither the specificCTSBvariants driving these associations nor the functional pathways that link catB to PD pathogenesis have been characterized. Cat...
Cathepsin B has previously been suggested to contribute to the degradation of α-syn and genetic variants in the CTSB locus are significantly associated with PD, suggesting that this lysosomal protease may play an important role in the disease. Here we provide genetic and functional evidence support...
We next tried to address lysosomal functions in NSCs in the juvenile and adult mouse brain. We monitored in vivo expression of the lysosomal proteins cathepsin B, cathepsin L, and Lamp1 by immunohistochemistry of the DG and the SVZ of the lateral ventricle. To identify NSCs in brain tissues,...
Cathepsin B/L activity. Cells were lysed 3 days after transfection in SCA lysis buffer (20 mM Hepes, 10 mM KCL, 1.5 mM EDTA, 1 mM EGTA and 250 mM sucrose) supplemented with 1 mM pefablock. For zFRase activity measurement, the lysate was mixed 1:1 with  2 cathepsin reaction ...
Cathepsin D: newly discovered functions of a long-standing aspartic protease in cancer and apoptosis. The lysosomal aspartic protease cathepsin D (cath-D) is over-expressed and hyper-secreted by epithelial breast cancer cells. This protease is an independen... E Liaudet-Coopman,M Beaujouin,D ...
Deficiency of the lysosomal cysteine protease cathepsin L (Ctsl) in mice results in a phenotype affecting multiple tissues, including thymus, epidermis, and hair follicles, and in the heart develops as a progressive dilated cardiomyopathy (DCM). To understand the role of Ctsl in the maintenance of...
Google Share on Facebook lysosome (redirected fromLysosomal enzymes) Thesaurus Medical Encyclopedia ly·so·some (lī′sə-sōm′) n. A membrane-bound organelle in the cytoplasm of most cells containing various hydrolytic enzymes that function in intracellular digestion. ...
The enzymatic assay revealed that trametinib increases cathepsin B activity in the presence of Aβ42 oligomers (Fig. 4J). Trametinib-mediated recovery of Aβ42-induced dendritic spine loss was prevented by lysosome and protease inhibitors, NH4Cl, and leupeptin in primary hippocampal neurons (...
Synergistic apoptotic response between valproic acid and fludarabine in chronic lymphocytic leukaemia (CLL) cells involves the lysosomal protease cathepsin B. Blood Cancer J 2013; 3: e153. 33. Jenkins RW, Canals D, Hannun YA. Roles and regulation of secretory and lysosomal acid sphingomyelinase...