Whole genome/transcriptome sequencing (WGS/WTS) provides comprehensive insights, while targeted sequencing is more cost-effective and sensitive. In comparison to short-read sequencing, which still dominates the field due to high speed and cost-effectiveness, long-read sequencing can overcome alignment ...
其中,precisionFDA的挑战框架是该平台最具开放性的功能之一,它支持在公开环境中举办生物数据挑战,并提供必要的资源以便进行测试和验证。 在2016年,首届瓶中基因组(GIAB)-precisionFDA真相挑战赛正式启幕,挑战参赛者从两个GIAB样本的短读长数据中识别出小变体。虽然HG001(也被称为NA12878)的基准测试数据已提前公布,但当...
RNA sequencing (RNA-seq) that utilises short-read next-generation sequencing (NGS) or long-read sequencing (such as PacBio and Oxford Nanopore Technologies sequencing) is a powerful tool in understanding transcriptional diversity and regulation during various biological processes, including hematopoiesis2...
Long-read Oxford Nanopore sequencing has democratized microbial genome sequencing and enables the recovery of highly contiguous microbial genomes from isolates or metagenomes. However, to obtain near-finished genomes it has been necessary to include shor
Sentieon | 每周文献-Long Read Sequencing(长读长测序)-第七期,研究者通过将PacBio长读长数据进行组装后对基因组进行repeat和gene注释。然后,利用测序或者从公开发表文献中下载的棉花基因组数据进行比对、变异检测、联合基因分型对亚洲栽培棉和非洲亚种进行比较。此过
PacBio incorporated the NVIDIA A100 Tensor Core GPU into its Revio system to accelerate the speed and accuracy of long‑read sequencing, while minimizing costs.
A modified Chromium 10x droplet-based protocol that subsamples cells for both short-read and long-read (nanopore) sequencing together with a new computational pipeline (FLAMES) is developed to enable isoform discovery, splicing analysis, and mutation det
The long and the short of it: unlocking nanopore long-read RNA sequencing data with short-read tools 长和短:用短读工具解锁纳米孔长读RNA测序数据 View ORCID ProfileXueyiDong,View ORCID ProfileLuyiTian,View ORCID ProfileQuentinGouil,HasaruKariyawasam,View ORCID ProfileShianSu,View ORCID ProfileRicar...
Given the broadening interest in long-read sequencing and the fast-paced development of applications and tools, the current review aims to provide a description of the guiding principles of long-read data analysis, a survey of the available tools for different tasks as well as a discussion of ...
Although the Rescue & Resolve algorithm was developed for use with long-read sequencing information, the algorithm could also be applied to proteogenomic databases and transcriptional abundance information derived from short-read sequencing approaches. The results and concepts described here provide a ...