Short-read sequencing is a powerful tool for generating genomic data. With short read sequencing, DNA or RNA can be sequenced in a shorter amount of time and at a lower cost than traditional methods. This technology has revolutionized biomedical research and led to important discoveries in genomic...
Iyer, Sara Goodwin, and William Richard McCombie Abstract: “Long-read sequencing technologies have improved the contiguity and, as a result, the quality of genome assemblies by generating reads long enough to span and resolve complex or … more »...
The present study provides a comprehensive resource of five BMI and DSE reproductive glands. As the dataset contains both long-read and short-read sequencing data, this rich sequencing dataset paves the avenue for quantifying gene or transcript expression, evaluating alternative splicing, delving novel...
RNA sequencing (RNA-seq) that utilises short-read next-generation sequencing (NGS) or long-read sequencing (such as PacBio and Oxford Nanopore Technologies sequencing) is a powerful tool in understanding transcriptional diversity and regulation during various biological processes, including hematopoiesis2...
Sentieon | 每周文献-Long Read Sequencing(长读长测序)-第七期,研究者通过将PacBio长读长数据进行组装后对基因组进行repeat和gene注释。然后,利用测序或者从公开发表文献中下载的棉花基因组数据进行比对、变异检测、联合基因分型对亚洲栽培棉和非洲亚种进行比较。此过
在2016年,首届瓶中基因组(GIAB)-precisionFDA真相挑战赛正式启幕,挑战参赛者从两个GIAB样本的短读长数据中识别出小变体。虽然HG001(也被称为NA12878)的基准测试数据已提前公布,但当时并未公开HG002的基准测试数据,这使得该挑战成为首个盲法种系变异检测挑战。其结果公开后,已被用作评估新变异检测方法的基准。尽管目...
Given the broadening interest in long-read sequencing and the fast-paced development of applications and tools, the current review aims to provide a description of the guiding principles of long-read data analysis, a survey of the available tools for different tasks as well as a discussion of ...
Targeted-hybridization short-read sequencing The Genomic DNA Extraction Kit (Vazyme, Nanjing, China) was used to extract genomic DNA according to the instructions of the manufacturer. The DNA concentration was quantified using the Qubit dsDNA HS Assay Kit (Thermo Fisher). 500 ng of DNA was divide...
README MIT license This repository contains the code used to perform the analysis and generate the figures in this paper: Benchmarking long-read RNA-sequencing analysis tools using in silico mixturesXueyi Dong, Mei R. M. Du, Quentin Gouil, Luyi Tian, Jafar S. Jabbari, Rory Bowden, Pedro ...
The long and the short of it: unlocking nanopore long-read RNA sequencing data with short-read tools 长和短:用短读工具解锁纳米孔长读RNA测序数据 View ORCID ProfileXueyiDong,View ORCID ProfileLuyiTian,View ORCID ProfileQuentinGouil,HasaruKariyawasam,View ORCID ProfileShianSu,View ORCID ProfileRicar...