By taking advantage of long-read sequencing, we detected clonal integration events which were defined as a collection of chimeric reads (> 5 kb) containing at least one fragment of HPV DNA wrapped on both sides by the human genome (Table1and Supplementary Table3). Nanopore technology is ...
Long-read Oxford Nanopore sequencing has democratized microbial genome sequencing and enables the recovery of highly contiguous microbial genomes from isolates or metagenomes. However, to obtain near-finished genomes it has been necessary to include shor
Long-Read Sequencing Reveals Genetic Adaptation of 来自 NCBI 喜欢 0 阅读量: 2 作者:A Thibau,K Hipp,DJ Vaca,S Chowdhury,J Malmstrm,A Saragliadis,W Ballhorn,D Linke,VAJ Kempf 关键词: Bartonella adhesin A PacBio SMRT sequencing genetic variability host matrix-pathogen interaction trimeric auto...
we used Pacific Biosciences single-molecule real-time (SMRT) long-read sequencing technology to generate transcripts from the Pacific white shrimp (Litopenaeus vannamei). We obtained 322,600 full-length non-chimeric reads, from which we generated 51,367 high-quality unique full-...
9,10 However, the MEI observed here in CDKL5 would likely be missed by exome sequencing as the breakpoints are intronic, and in fact it was also missed in our previous short-read genome sequencing analysis.15 Global analyses of MEIs, such as our assessment of de novo Alu insertion rates...
Keywords:hybrid assembly, bacterial genomics, long-read sequencing,Enterobacteriaceae, plasmid assembly 摘要 Illumina测序允许快速、廉价和准确的全基因组细菌分析,但是短读(300 bp)通常不能实现完整的基因组组装。 长读测序大大有助于解决复杂的细菌基因组,特别是当结合短读Illumina数据(杂交组装)。
A modified Chromium 10x droplet-based protocol that subsamples cells for both short-read and long-read (nanopore) sequencing together with a new computational pipeline (FLAMES) is developed to enable isoform discovery, splicing analysis, and mutation det
The human transcriptome annotation is regarded as one of the most complete of any eukaryotic species. However, limitations in sequencing technologies have biased the annotation toward multi-exonic protein coding genes. Accurate high-throughput long read
Carex L., a grass genus commonly known as sedges, is distributed worldwide and contributes constructively to turf management, forage production, and ecological conservation. The development of next-generation sequencing (NGS) technologies has considerabl
Background Deregulated gene expression is a hallmark of cancer; however, most studies to date have analyzed short-read RNA sequencing data with inherent limitations. Here, we combine PacBio long-read isoform sequencing (Iso-Seq) and Illumina paired-end short-read RNA sequencing to comprehensively ...