Long-read Oxford Nanopore sequencing has democratized microbial genome sequencing and enables the recovery of highly contiguous microbial genomes from isolates or metagenomes. However, to obtain near-finished genomes it has been necessary to include shor
By taking advantage of long-read sequencing, we detected clonal integration events which were defined as a collection of chimeric reads (> 5 kb) containing at least one fragment of HPV DNA wrapped on both sides by the human genome (Table1and Supplementary Table3). Nanopore technology is ...
long-read RNA sequencingpervasive transcriptionnoncoding RNAorganelle genomemitochondrial transcriptionplastid transcription40 years ago, organelle genomes were assumed to be streamlined and, perhaps, unexciting remnants of their prokaryotic past. However, the field of organelle genomics has exposed an ...
we used Pacific Biosciences single-molecule real-time (SMRT) long-read sequencing technology to generate transcripts from the Pacific white shrimp (Litopenaeus vannamei). We obtained 322,600 full-length non-chimeric reads, from which we generated 51,367 high-quality unique full-...
Out of the many pathogenic bacterial species that are known, only a fraction are readily identifiable directly from a complex microbial community using standard next generation DNA sequencing. Long-read sequencing offers the potential to identify a wider range of species and to differentiate between st...
Keywords:hybrid assembly, bacterial genomics, long-read sequencing,Enterobacteriaceae, plasmid assembly 摘要 Illumina测序允许快速、廉价和准确的全基因组细菌分析,但是短读(300 bp)通常不能实现完整的基因组组装。 长读测序大大有助于解决复杂的细菌基因组,特别是当结合短读Illumina数据(杂交组装)。
来自 NCBI 喜欢 0 阅读量: 342 作者:M Boetzer,W Pirovano 摘要: The recent introduction of the Pacific Biosciences RS single molecule sequencing technology has opened new doors to scaffolding genome assemblies in a cost-effective manner. The long read sequence information is promised to enhance the...
Single-cell RNA sequencing predominantly employs short-read sequencing to characterize cell types, states and dynamics; however, it is inadequate for comprehensive characterization of RNA isoforms. Long-read sequencing technologies enable single-cell RNA isoform detection but are hampered by lower throughpu...
Here, we integrate single-molecule long-read sequencing with short-read sequencing to detect sperm intact RNAs (spiRNAs). We identify 3440 spiRNA species in mice and 4100 in humans. The spiRNA profile consists of both mRNAs and long non-coding RNAs, is evolutionarily conserved between mice ...
Single-cell nanopore sequencing of full-length mRNAs transforms single-cell multi-omics studies. However, challenges include high sequencing errors and dependence on short-reads and/or barcode whitelists. To address these, we develop scNanoGPS to calcula