Long-read sequencing, Nanopore sequencing, Copy number variation, Read-counting applicationsWe present SMURF-seq, a protocol to efficiently sequence short DNA molecules on a long-read sequencer by randomly ligating them to form long molecules. Applying SMURF-seq using the Oxford Nanopore MinION yields...
PacBio’s Sequel IIe long-read sequencer was designed with CPU-based computation. While functional, it reached a performance threshold that limited optimal throughout and, therefore, its utility for commercial customers. To address this limitation, PacBio introduced the Revio System featuringNVIDIA A10...
Long-Read Sequencing Microarray Technology Sequencing Method Explorer All Technologies Next-generation sequencing for beginners Curious about using NGS in your research? Learn the basics of next-generation sequencing and find tips for getting started. Learn about NGS Illumina Research & Innovation Geno...
Many genomes have been sequenced to high-quality draft status using Sanger capillary electrophoresis and/or newer short-read sequence data and whole genome... Yu-Min,Lin,Chin-Jung,... - 《Plos One》 被引量: 636发表: 2012年 Advantages of genome sequencing by long-read sequencer using SMRT ...
1D (SQK-LSK109). DNA was purified between each step using Agencourt AMPure XP beads (A63882, Beckman Coulter) and was quantified via a Qubit Fluorometer 2.0 (ThermoFisher Scientific, Waltham, MA). The prepared libraries were sequenced using a PromethION sequencer and 1D flow cell with ...
Cancer is a disease largely caused by genomic aberrations. Utilizing many rapidly emerging sequencing technologies, researchers have studied cancer genomes to understand the molecular statuses of cancer cells and to reveal their vulnerabilities, such as
README License Caution DAJIN is deprecated. Please use DAJIN2, which has become the successor to DAJIN. 日本語はこちら DAJIN is a genotyping software for genome-edited organisms using Nanopore sequencer. DAJIN is named after 一網打尽 (Ichimou DAJIN or Yī Wǎng Dǎ jìn), meaning catching...
HiSeq. 2500 high-throughput sequencer. Raw sequencing reads were quality controlled to remove adaptor sequences, low-quality reads (reads where quality was ≤10% for >50% of all nucleotides), and read with many unknown nucleotides (>10%). Cleaned sequences were used for SMRT error correction...
the smallest physical sequencer, is smaller than a typical stapler and can run both MinION and Flongle flow cells. A unique feature of ONT sequencing is portability in that the smaller devices, such as the Flongle or MinION, can be powered by a laptop, allowing them to be used in isola...
Targeted phasing of 2–200 kilobase DNA fragments with a short-read sequencer and a single-tube linked-read library method Article Open access 05 April 2024 Refined detection and phasing of structural aberrations in pediatric acute lymphoblastic leukemia by linked-read whole-genome sequencing Arti...