The liver is the largest solid organ in the body and is critical for metabolic and immune functions. However, little is known about the cells that make up the human liver and its immune microenvironment. Here we report a map of the cellular landscape of the human liver using single-cell RN...
used to capture single cells in oil droplets through microfluidic chips, e.g., 10X chromium. The core idea is to assign different barcode sequences to different cells [52]. When building a library, nucleic acid molecules with the same barcode sequence are considered to be from the same cell...
In addition, single-cell approaches are starting to dissect key cellular and molecular functions in liver cancer. In the near future, new techniques such as spatial transcriptomics and multiomic approaches will further deepen our understanding of disease pathogenesis, enabling the identification of novel...
Together, our study presents a comprehensive view of the human liver at single-cell resolution that outlines the characteristics of resident cells in the liver, and in particular provides a map of the human hepatic immune microenvironment. 展开 ...
空间分选可作为一种普遍适用的策略,结合高灵敏、高深度的单细胞蛋白质组学(single-cell proteomics),将为生物系统中细胞异质性研究、精细蛋白分子图谱构建、空间蛋白质网络分析等提供最本质的精准信息,为肿瘤异质性、干细胞分化、生殖细胞发育等提供新的思路和见解。
For P. vivax, single-cell transcriptomic studies have been conducted in blood stages collected from infected monkeys (Sà et al., 2020), but the analysis of liver infection has not been performed to date. Here, we present a comprehensive view of the liver-stage transcriptomes of a human-...
The human liver is an essential multifunctional organ. The incidence of liver diseases is rising and there are limited treatment options. However, the cellular composition of the liver remains poorly understood. Here we performed single-cell RNA sequencing of about 10,000 cells from normal liver ti...
Here, we dissect the diversity of LrICs by a comprehensive transcriptomic profiling using the unbiased single-cell RNA-sequencing (scRNA-seq). A total of 70, 706 of CD45immune cells from the paired liver perfusion, spleen and peripheral blood as references were profiled. We identified more ...
Landscape of Infiltrating T Cells in Liver Cancer Revealed by Single-Cell Sequencing 这篇文章[1]的一个亮点在于对肿瘤中的T细胞有了认识。 data 不得不说,这个样本的数量和质量真的太好了,一共是六个病人的外周血,正常组织,癌症区域配对样本,形成了非常好的对照。不知道是不是因为P1202病人T细胞的数量太少...
Cell Research, 2021年8月2日 8月2日,北京大学生命科学学院李程团队,与中国科学院动物研究所刘峰团队合作,在期刊 Cell Research 发表题为 Identification of HSC/MPP expansion units in fetal liver by single-cell spatiotemporal transcriptomics 的论文。北京大学生命科学学院石强博士,和中国科学院动物研究所博士研究...