Therefore, the objective of this study is to provide a comprehensive overview of the knowledge structure and research hotspots in the field of single-cell sequencing in liver cancer research through the use of bibliometrics. Method: Publications related to the application of single-cell sequencing ...
6、对肿瘤区域的克隆型T细胞进行了分析,发现危重病人的克隆型Treg占据的比例更高,同时发现了这样一个亚群CD8+FOXP3+,分析克隆发现是,CD8+FOXP3-的细胞分化而来, Tregs and exhausted CD4 T cell clusters 共享很少,indicated the potential conversion from exhausted CD4 T cells to inducible Tregs。 最后看一下文...
但是目前治疗手段有限且免疫治疗缺乏临床成功。 3. 参与肿瘤发生发展的免疫细胞类型多样且异质性很强,只有对浸润淋巴细胞进行单细胞测序分析才能更好的理解复杂的肿瘤微环境,真正获得系统详尽的肿瘤免疫图谱,从而有助于肿瘤免疫疗法的发展及有效靶点和生物标志物的发现。 研究策略: 对六位未进行治疗的肝癌病人的肿瘤样本...
The liver is the largest solid organ in the body and is critical for metabolic and immune functions. However, little is known about the cells that make up the human liver and its immune microenvironment. Here we report a map of the cellular landscape of the human liver using single-cell RN...
Primary liver cancer (PLC) poses a leading threat to human health, and its treatment options are limited. Meanwhile, the investigation of homogeneity and heterogeneity among PLCs remains challenging. Here, using single-cell RNA sequencing, spatial transc
Liver cancer arises from the evolutionary selection of the dynamic tumor microenvironment (TME), in which the tumor cell generally becomes more heterogeneous; however, the mechanisms of TME-mediated transcriptional diversity of liver cancer remain unclear. Here, we assess transcriptional diversity in 15...
BF16F10 human melanoma cell line CNE-2 human nasopharyngeal cancer cell line F3II murine carcinoma cell line HaCaT human keratinocytes HBL-100 human breast cancer cell line HCT-116 human colon carcinoma cancer cell line HeLa human cervical cancer cell line HepG2 human liver cancer cell line HL...
Analysis of Liver Cancer Cell Lines Identifies Agents With Likely Efficacy Against Hepatocellular Carcinoma and Markers of Response.
Here we performed single-cell RNA-sequencing (scRNA-seq) analysis of 189 samples collected from 124 patients and 8 mice with liver cancer. With more than 1 million cells analysed, we stratified patients into five TIME subtypes, including immune activation, immune suppression mediated by myeloid ...
Multiregional single-cell dissection of tumor and immune cells reveals stable lock-and-key features in liver cancer Multiregion sequencing is needed to better capture the heterogeneity of hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (iCCA). Here, the authors analyse HCC and iCCA tu...