little is known about the cells that make up the human liver and its immune microenvironment. Here we report a map of the cellular landscape of the human liver using single-cell RNA sequencing. We provide the transcriptional profiles of 8444 parenchymal and non-parenchymal...
Here, single-cell RNA-sequencing was used to unravel the heterogeneity of human liver cells, in particular of hepatocytes (HEPs) and hepatic stellate cells (HSCs).#The transcriptome of ~25,000 freshly isolated human liver cells was profiled using droplet-based RNA-sequencing. Recently published ...
Here, we dissect the diversity of LrICs by a comprehensive transcriptomic profiling using the unbiased single-cell RNA-sequencing (scRNA-seq). A total of 70, 706 of CD45immune cells from the paired liver perfusion, spleen and peripheral blood as references were profiled. We identified more ...
6、对肿瘤区域的克隆型T细胞进行了分析,发现危重病人的克隆型Treg占据的比例更高,同时发现了这样一个亚群CD8+FOXP3+,分析克隆发现是,CD8+FOXP3-的细胞分化而来, Tregs and exhausted CD4 T cell clusters 共享很少,indicated the potential conversion from exhausted CD4 T cells to inducible Tregs。 最后看一下文...
The human liver is an essential multifunctional organ. The incidence of liver diseases is rising and there are limited treatment options. However, the cellular composition of the liver remains poorly understood. Here we performed single-cell RNA sequencing of about 10,000 cells from normal liver ti...
Here we report a map of the cellular landscape of the human liver using single-cell RNA sequencing. We provide the transcriptional profiles of 8444 parenchymal and non-parenchymal cells obtained from the fractionation of fresh hepatic tissue from five human livers. Using gene expression patterns, ...
The human liver is an essential multifunctional organ. The incidence of liver diseases is rising and there are limited treatment options. However, the cellular composition of the liver remains poorly understood. Here we performed single-cell RNA sequencing of about 10,000 cells from normal liver ti...
Single-cell RNA sequencing reveals the pro-inflammatory roles of liver-resident Th1-like cells in primary biliary cholangitis 01 June 2020 Introduction The mammalian liver is a complex organ consisting of diverse cell types that perform multiple physiological functions including digestion, synthesis, metab...
single-cell technology, we can now decipher heterogeneity, differentiation, and intercellular communication at the single-cell level in sophisticated organs and complicated diseases. For example, single-cell RNA sequencing (scRNA-seq) can characterise the complete transcriptome of a single cell on a ...
单细胞测序显示肝癌中浸润T细胞的景观图(Landscape of Infiltrating T Cells in Liver Cancer Revealed by Single-Cell Sequencing) 科学问题: 1.在过去的十年里,免疫治疗显著的改善了癌症治疗的状况,但对于不同的肿瘤病人或肿瘤类型,它的效果是不一致的。突变数量、肿瘤浸润的淋巴细胞和药物靶点表达等若干因素,虽然与...