Single-Cell RNA Sequencing Identifies Crucial Genes Influencing the Polarization of Tumor-Associated Macrophages in Liver Cancerdoi:10.1155/2024/7263358RNA sequencingLIVER cancerTRANSCRIPTION factorsGENE expressionGENESBackground. In the context of hepatocellular carcinoma (HCC), tumor-associated macrop...
Single-cell RNA sequencing (scRNA-seq), a technology that analyzes transcriptomes of complex tissues at single-cell levels, can identify differential gene expression and epigenetic factors caused by mutations in unicellular genomes, as well as new cell-specific markers and cell types. scRNA-seq plays...
lysis, reverse transcription (RT), complementary DNA (cDNA) amplification, library construction, sequencing, and data analysis [16] (Fig.1). Although capturing single cells quickly and accurately with high efficiency may seem trivial, it is one of the main challenges of single-cell sequencing...
lysis, reverse transcription (RT), complementary DNA (cDNA) amplification, library construction, sequencing, and data analysis [16] (Fig.1). Although capturing single cells quickly and accurately with high efficiency may seem trivial, it is one of the main challenges of single-cell sequencing...
healthy human liver with single-cell RNA sequencing (scRNA-seq). Although scRNA-seq is a powerful tool for describing highly heterogeneous cell populations such as those found in whole tissue2,3, it has not yet been widely applied to describe whole human organs, with only maps of isolated isl...
Single-cell technologies have the capacity to unravel the phenotypes of individual cells within a tissue, revolutionizing our understanding of cancer biology. Single-cell RNA sequencing (scRNA-seq), involving the main steps of single-cell isolation, cell lysis for mRNA capture, reverse transcription,...
Breast cancer is the most common cancer affecting women across the world. Tumor endothelial cells (TECs) and malignant cells are the major constituents of the tumor microenvironment (TME), but their origin and role in shaping disease initiation, progress
This insightful three-part webinar, explores the impact of single cell sequencing in advancing cancer diagnosis and treatment. Talk 1: Comprehensive profiling of tumour heterogeneity and its microenvironment in advanced non-small cell lung cancer at single cell resolution. In this first section, Prof...
Primary liver cancer (PLC) poses a leading threat to human health, and its treatment options are limited. Meanwhile, the investigation of homogeneity and heterogeneity among PLCs remains challenging. Here, using single-cell RNA sequencing, spatial transc
? 2024 Elsevier LtdHepatocellular carcinoma (HCC) is the most common type of liver cancer, and the high heterogeneity of its tumor immune microenvironment (TME) is often a key reason for poor treatment response. To investigate the heterogeneity of the TME in HCC and its potential underlying mech...