Single-cell technologies have the capacity to unravel the phenotypes of individual cells within a tissue, revolutionizing our understanding of cancer biology. Single-cell RNA sequencing (scRNA-seq), involving the main steps of single-cell isolation, cell lysis for mRNA capture, reverse transcription, ...
Single-Cell RNA Sequencing: Technological Progress and Biomedical Application in Cancer Research.Single-cell RNA sequencingTumor heterogeneityMicroenvironmentLineage tracingPersonalized medicineSingle-cell RNA-seq (scRNA-seq) is a revolutionary technology that allows for the genomic investigation of individual ...
Single-cell RNA sequencing (scRNA-seq), a technology that analyzes transcriptomes of complex tissues at single-cell levels, can identify differential gene expression and epigenetic factors caused by mutations in unicellular genomes, as well as new cell-specific markers and cell types. scRNA-seq plays...
Instead of isolating single cells within physical compartments, single-cell combinational indexing RNA sequencing (sci-RNA-seq) in 2017 was successively developed using a two-step combinatorial indexing strategy, a method using split-pool barcoding of nucleic acids to uniquely label a large number of ...
Bone Research www.nature.com/boneres REVIEW ARTICLE OPEN Single-cell RNA sequencing in orthopedic research Tao Wang1,2, Ling Wang2,3, Liping Zhang4, Yubin Long1,2, Yingze Zhang1,2,5 and Zhiyong Hou1,2,5 ✉ Although previous RNA sequencing methods have been widely used in orthopedic ...
Methodologies of single-cell RNA-seq 2. Single-cell isolation techniques 3. Single-cell RNA-seq in solid tumor research 4. Single-cell RNA-seq in circulating tumor cell research 5. PERSPECTIVES 展开 关键词: single cell, RNA sequencing, tumor, circulating tumor cell ...
Advances of individual-cell RNA sequencing in carcinoma Immune cells Non-immune cells Combination of scRNA-seq and other methods Use of individual-cell RNA sequencing inside different fields Conclusions and perspective Author contributions Declaration of interests ReferencesShow full outline Cited by (47)...
Although previous RNA sequencing methods have been widely used in orthopedic research and have provided ideas for therapeutic strategies, the specific mechanisms of some orthopedic disorders, including osteoarthritis, lumbar disc herniation, rheumatoid a
Briefly, analysis of single-cell RNA sequencing data can be performed for the following purposes: finding common cell types and cell subtypes among different tumor samples, identifying cancer cells markers that are unique in comparison to normal cells, analysis of pseudo-temporal trajectories to ...
Single-cell RNA sequencing (scRNA-seq) is a powerful technology for cancer research. Previous scRNA-seq studies have reported the complexity of the tumor microenvironment in iCCAs without taking into consideration of the histological classification, which may not accurately reflect the diversity of this...