Single-cell technologies have the capacity to unravel the phenotypes of individual cells within a tissue, revolutionizing our understanding of cancer biology. Single-cell RNA sequencing (scRNA-seq), involving the main steps of single-cell isolation, cell lysis for mRNA capture, reverse transcription, ...
Single-Cell RNA Sequencing: Technological Progress and Biomedical Application in Cancer Research.Single-cell RNA sequencingTumor heterogeneityMicroenvironmentLineage tracingPersonalized medicineSingle-cell RNA-seq (scRNA-seq) is a revolutionary technology that allows for the genomic investigation of individual ...
Single-cell RNA sequencing (scRNA-seq), a technology that analyzes transcriptomes of complex tissues at single-cell levels, can identify differential gene expression and epigenetic factors caused by mutations in unicellular genomes, as well as new cell-specific markers and cell types. scRNA-seq plays...
Single-cell sequencing in cancer research Genome-wide single-sequencing investigations have the potential to classify individual within a mass. In recent years, various single-DNA and RNA quantific... M Mato Prado,AE Frampton,J Stebbing,... - ...
and the ability to study relationships among cells or cell types [12]. Single-cell RNA sequencing (scRNA-seq) was first reported in 2009 in a study profiling the transcriptome at single-cell resolution, and scRNA-seq is gradually becoming a popular tool used in human cancer research for eluci...
Advances of individual-cell RNA sequencing in carcinoma Immune cells Non-immune cells Combination of scRNA-seq and other methods Use of individual-cell RNA sequencing inside different fields Conclusions and perspective Author contributions Declaration of interests ReferencesShow full outline Cited by (47)...
Bone Research www.nature.com/boneres REVIEW ARTICLE OPEN Single-cell RNA sequencing in orthopedic research Tao Wang1,2, Ling Wang2,3, Liping Zhang4, Yubin Long1,2, Yingze Zhang1,2,5 and Zhiyong Hou1,2,5 ✉ Although previous RNA sequencing methods have been widely used in orthopedic ...
et al. Analyzing tumor heterogeneity and driver genes in single myeloid leukemia cells with SBCapSeq. Nat. Biotechnol. 34, 962–972 (2016). Article CAS PubMed PubMed Central Google Scholar Faridani, O. R. et al. Single-cell sequencing of the small-RNA transcriptome. Nat. Biotechnol. 34...
This insightful three-part webinar, explores the impact of single cell sequencing in advancing cancer diagnosis and treatment. Talk 1: Comprehensive profiling of tumour heterogeneity and its microenvironment in advanced non-small cell lung cancer at single cell resolution. In this first section, Prof...
cell RNA sequencing (scRNA-seq) technique has introduced a new era of research on these topics, as this method provides information regarding cellular heterogeneity, new cell subtypes, functions of novel subclusters, potential molecular mechanisms, cell-fate transitions, and cell‒cell interactions ...