c, Fatty acid profile by GC–MS following ash-2 depletion. Fatty acid levels were normalized to the control condition. Data are the mean ± s.d. of three independent experiments, each with three biological replicates. Significant P values are shown. P values: two-way ANOVA with ...
In this work, lipid profile migration from muscle to juice during the tilapia muscle steaming process was revealed by a transactional analysis of data from ultra-high-performance liquid chromatography coupled with Q Exactive (UHPLC-QE) Orbitrap mass spectrometry (MS) and lipidomics. Firstly, the ...
Subsequently, the developed method was applied to profile the wide changes of metabolites and lipids in the frontal cortex and hippocampus of T1D mice with cognitive decline and to investigate potential molecular mechanisms underlying diabetic cognitive impairment. Materials and methods Reagents and ...
69. Duda JE, Giasson BI, Gur TL, Montine TJ, Robertson D, Biaggioni I, Hurtig HI, Stern MB, Gollomp SM, Grossman M: Immunohistochemical and biochemical studies demonstrate a distinct profile of [alpha]-synuclein permutations in multiple system atrophy. J Neuropathol Exp Neurol 2000, 59(9)...
We profiled a total of 10,042 QC-positive cells by single-cell RNA-seq, resulting in 133 metacells (Data S4). Remarkably, the monocyte-macrophage compartment of Trem2−/− mice did not fully progress toward the HFD-associated state observed in wild-type littermates. Instead, these ...
(Additional file TableS4). In the lipid profile, we have observed a significant increase in levels of GPL and SL, both categories similarly contributing to the CSF changes. Further univariate analysis revealed significant alterations in the levels of PC, PE, and their respective lysolipids. ...
Transcriptional profile analyzed by RNA-Seq showed that expression levels of 1213 genes (including key carbon fixation, TCA cycle, glycerolipid metabolism and nitrogen assimilation genes) increased, with a false discovery rate cut-off of 0.001, under N deprivation. However, most light harvesting ...
Green color indicates a perfect match, red—a mismatch, and gray—a non-contradictory alternative assignment. Color intensity reflects the number of overlapping lipids. j Distributions of the correlation coefficients for lipid intensity profile comparisons between humans (n = 4 individuals) and ...
tube for single-photon detection and a signal processing circuit for time-resolved measurement. Using the nonlinear least-squares method, the digitized temporal profile data from an in vitro sample or tissue was fitted with a theoretical temporal profile, derived from the analytical solution of the ...
A profile (a) and centroid (b) version of the same spectrum. The profile raw data detected by a mass spectrometer consists of distributed signal across m/z values at each point where an ion is detected. Centroid data is raw data that has been processed by an algorithm to retain only the...