Further data processing was done using an in-house analysis pipeline written in R (version 3.6.3; available in GitHub at https://github.com/brunetlab). Briefly, processing for samples and spike-in standards were done in the same way. All ions for one lipid were aggregated and lipids with...
The work of Michael Levin @Tufts, The Levin Lab Michael Levin is an American developmental and synthetic biologist at Tufts University, where he is the Vannevar Bush Distinguished Professor. Levin is a director of the Allen Discovery Center at Tufts University and Tufts Center for Regenerative and...
The light-dark cycle was 12 hours each and the mice were fed ad libitum food (Lab Diet 5002 Certified Rodent Diet) and water. Mice were euthanized by cervical dislocation (>PND14) or decapitation (PND7) and exsanguinated via an incision in the left ventricle. Dissection and ...
Scripts reproducing the analysis will be available at: https://bitbucket.org/amitlab/. Acknowledgments We thank Genia Brodsky from the Scientific Illustration unit of the Weizmann Institute for artwork, Tomer Meir Salame from the Cell Cytometry unit, Calanit Raanan and Marina Cohen for histology, ...
Arch Pathol Lab Med. 2019;143(11):1317–26. https://doi.org/10.5858/arpa.2019-0338-RA. Article CAS PubMed PubMed Central Google Scholar Li Y, Zhao L, Li XF. Hypoxia and the tumor microenvironment. Technol Cancer Res Treat. 2021;20:15330338211036304. https://doi.org/10.1177/...
All procedures employed analytical-grade chemicals that were readily available in the lab. 3. Methods 3.1. Selection of solid-liquid lipids For the selection of lipids, accurately 0.1 g of drug taken in a test tube in which a sufficient quantity of solid lipid (SL) was steadily mixed until ...
The values of −log10(p value) were obtained from the Harmonizome database (https://maayanlab.cloud/Harmonizome/gene/TFAP4). Error bars indicate mean ± SEM. Statistical analyses were performed by Student’s t test (unpaired, two tailed), *p < 0.05; **p < 0.01; ***p...
RNA reads were aligned and mapped to the mm9 mouse reference genome by TopHAEC2 (version 2.0.11) (https://ccb.jhu.edu/software/tophat/index.shtml)59, and transcriptome reconstruction was performed by Cufflinks (version 2.1.1) (https://cole-trapnell-lab.github.io/cufflinks/), with gene ...
Colorimetric analyses of lipids were measured at 570 nm on an Omega FLOUstar 96-well plate reader from BMG labtech (Ortenberg, Germany). Serum β-hydroxybutyrate measurements were determined from mouse serum samples using a β-hydroxybutyrate LiquiColor kit (Stanbio Laboratory, Boerne, TX, USA)...
(Vikari, 1976, J. Clin. Lab. Invest. 36:265 hereby incorporated by reference). Subclasses of HDL can be separated from one another and from other classes of lipoprotein, see e.g., Whitaker et al., 1986, Clin. Chem. 32:1274, hereby incorporated by reference, or Gidez et al., 1982...