CEA was originally described as a colon-cancer-associated antigen, and tests for blood levels of CEA antigen are used to clinically monitor the progression of colon cancer. There are about 20 related proteins known; their genes are clustered on human chromosome 19, they belong to the Ig ...
DNA samples were extracted from AEC2 cells obtained from SftpcCreERT2+/−, Mfn1iΔAEC2, Mfn1/2iΔAEC2, controltdTomato-AEC2, and Mfn1iΔAEC2/tdTomato-AEC2 mice using DNeasy blood and tissue kit (Qiagen), and were used for genotyping through PCR reactions and the subsequent resolution...
Research supported by grant U01 HL122703 from the National Heart Lung Blood Institute of NIH. Omics analyses were performed in the Environmental Molecular Sciences Laboratory, a national scientific user facility sponsored by the US. Department of Energy and located at Pacific Northwest National Laborator...
Concentration of insulin in the blood serum Concentrations of insulin (Ultra-sensitive mouse insulin ELISA kit, Crystal Chem,#90080) in the serum were measured using ELISA according to the manufacturer’s instructions. Quantification and Statistical Analysis Single-Cell RNA-Sequencing Analyses MARS-seq ...
On the contrary, oxidized cholesterol derivatives can easily diffuse across the blood brain barrier and may pro- vide a mechanism of interaction between central and peripheral cholesterol pools. These derivatives include 24S-hydroxycholesterol, which appears to be involved chiefly in excretion of excess ...
Labeling cell surface markers such as CD44 (breast)6, CD133 (bone)7, CD34 (blood)8, Lgr5 (colon)9 etc., is a phenotypic method for CSC enrichment. Monitoring anchorage-independent growth in vitro or tumor formation in immune-compromised mice in vivo are assays typically used for functional...
However, they only occur with a frequency of 0.2% when compared with other nucleated blood cells, making them difficult to study. The entire process of megakaryopoiesis is accompanied by a substantial membrane reorganization, including shaping a lobulated nuclear envelope, packing granules, generating...
Blood lipid disorder or dyslipidemia is a key component for the medical care of patients with cardiovascular disease (CVD). Dietary lipids on the risk of CVD have traditionally been revealed by their effects on low density lipoprotein cholesterol (LDL-C). This is a cross-over study to evaluate...
Methods of the invention use magnetically responsive particles for the rapid removal of lipoproteins that have been selectively precipitated from an aqueous medium such as blood, plasma, or serum. Preferred embodiments provide enhanced fractionation by selective precipitation of lipoproteins of one or more...
In one embodiment, sufferers of atopic dermatitis have high serum levels of reaginic (IgE) antibodies, peripheral eosinophilia, high levels of cAMP phosphodiesterase in their white blood cells, or a combination thereof. In one embodiment, symptoms of atopic dermatitis in infants comprise red, ...