Blood samples from the median cubital vein were drawn by smooth venipuncture employing minimal stasis and stored in siliconized glass tubes with ethylenediaminetetraacetic acid (VenoJect; Terumo, Tokyo, Japan). Plasma for nontargeted metabolomic profiling was obtained after centrifugation at 3000 ×...
CEA was originally described as a colon-cancer-associated antigen, and tests for blood levels of CEA antigen are used to clinically monitor the progression of colon cancer. There are about 20 related proteins known; their genes are clustered on human chromosome 19, they belong to the Ig ...
Establishment and maintenance of pregnancy depends on progesterone synthesized by luteal tissue in the ovary. Our objective was to identify the characteristics of lipid droplets (LDs) in ovarian steroidogenic cells. We hypothesized that LDs are a major f
However, the fact remains that cholesterol-bearing lipoproteins in the peripheral circulation do not cross the blood brain barrier and most brain cholesterol is synthesized in situ [115]. On the contrary, oxidized cholesterol derivatives can easily diffuse across the blood brain barrier and may pro-...
Glucose tolerance test Mice were fasted for 14 h and subsequently given 200 μl of a 0.1 g/ml (10%) glucose solution (JT Baker) by intraperitoneal injection. Blood glucose was determined at 0, 15, 45, 30, 60, 90 and 120 min post injection (Contour blood glucose meter, Baye...
QIAGEN 965152 QIAamp DNA Blood BioRobot MDx Kit (12) QIAGEN 962241 QIAprep 96 Plus BioRobot Kit (4) QIAGEN 51204 FlexiGene DNA Kit (50) QIAGEN 9015484 Reagent Holder, 6-bottle, 30 ml QIAGEN 9015474 Cable bare, P QIAGEN 9015472 Motor, Y ...
Blood lipid disorder or dyslipidemia is a key component for the medical care of patients with cardiovascular disease (CVD). Dietary lipids on the risk of CVD have traditionally been revealed by their effects on low density lipoprotein cholesterol (LDL-C). This is a cross-over study to evaluate...
Research supported by grant U01 HL122703 from the National Heart Lung Blood Institute of NIH. Omics analyses were performed in the Environmental Molecular Sciences Laboratory, a national scientific user facility sponsored by the US. Department of Energy and located at Pacific Northwest National Laborator...
However, they only occur with a frequency of 0.2% when compared with other nucleated blood cells, making them difficult to study. The entire process of megakaryopoiesis is accompanied by a substantial membrane reorganization, including shaping a lobulated nuclear envelope, packing granules, generating...
Blood retainability of a liposome can be enhanced by modifying the surface of the lipid membrane structure as the liposome with a hydrophilic polymer such as polyalkylene glycol. This means is described in, for example, Japanese Patent Unexamined Publication (KOKAI) Nos. 1-249717, 2-149512, 4-...