Kim, H., Zenhausern, R., Gentry, K.et al.Lipid nanoparticle-mediated mRNA delivery to CD34+cells in rhesus monkeys.Nat Biotechnol(2024). https://doi.org/10.1038/s41587-024-02470-2 Download citation Received15 April 2024 Accepted15 October 2024 ...
To function in vivo, mRNA requires safe, effective and stable delivery systems that protect the nucleic acid from degradation and that allow cellular uptake and mRNA release. Lipid nanoparticles have successfully entered the clinic for the delivery of mRNA; in particular, lipid nanoparticle–mRNA ...
In this Review, we briefly overview representative lipid nanoparticles used for mRNA delivery and describe key steps in the preclinical development of lipid nanoparticle–mRNA formulations, including the overcoming of physiological barriers, different administration routes, manufacturing and safety profiles. Fi...
Lipid nanoparticles (LNPs) for mRNA delivery have advanced significantly, but LNP-mediated DNA delivery still faces clinical challenges. This study compared various LNP formulations for delivering DNA-encoded biologics, assessing their expression efficacy and the protective immunity generated by LNP-encapsula...
Messenger RNA (mRNA) stands as a versatile instrument in this context, with its capacity to encode tumor-associated antigens (TAAs), immune cell receptors, cytokines, and antibodies. Nevertheless, the inherent structural instability of mRNA requires the development of effective delivery systems. Lipid...
Middle: EpCAM-CD3-hFc mRNA-LNP-treated T cells significantly decrease tumor growth. Right: Tumor size and weight are significantly decreased with EpCAM-CD3-hFc mRNA-LNP-treated T cells. See all the data in the paper “mRNA-Lipid Nanoparticle (LNP) Delivery of Humanized EpCAM-CD3 Bispecific ...
内容提示: Chemistry of Lipid Nanoparticles for RNA DeliveryPublished as part of the Accounts of Chemical Research special issue “mRNA Therapeutics”.Yulia Eygeris, ∥ Mohit Gupta, ∥ Jeonghwan Kim, and Gaurav Sahay*Cite This: https://doi.org/10.1021/acs.accounts.1c00544 Read OnlineACCESS...
Lipid nanoparticles have successfully entered the clinic for the delivery of mRNA; in particular, lipid nanoparticle–mRNA vaccines are now in clinical use against coronavirus disease 2019 (COVID-19), which marks a milestone for mRNA therapeutics. In this Review, we discuss the design of lipid ...
Lipid nanoparticles (LNPs) for the efficient delivery of drugs need to be designed for the particular administration route and type of drug. Here we report the design of LNPs for the efficient delivery of therapeutic RNAs to the lung via nebulization. We
Lipofectamine-mRNA complexes were formed by diluting Lipofectamine MessengerMax (5% v/v) and mRNA (25 µg mL−1) in PBS and incubating for 10 min at 22 °C. Nanoparticle size was measured using dynamic light scattering with Zetasizer Nano ZPS (Malvern). Preparing washed platelets ...