Kim, H., Zenhausern, R., Gentry, K.et al.Lipid nanoparticle-mediated mRNA delivery to CD34+cells in rhesus monkeys.Nat Biotechnol(2024). https://doi.org/10.1038/s41587-024-02470-2 Download citation Received15 April 2024 Accepted15 October 2024 ...
We report an LNP named LNP 67 that delivers mRNA to murine HSCs in vivo, primary human HSCs ex vivo and CD34 + cells in rhesus monkeys ( Macaca mulatta ) in vivo at doses of 0.25 and 0.4mgkg 1 . Without mobilization and conditioning, LNP 67 can mediate delivery of mRNA to HSCs and...
Lipid nanoparticles (LNPs) for mRNA delivery have advanced significantly, but LNP-mediated DNA delivery still faces clinical challenges. This study compared various LNP formulations for delivering DNA-encoded biologics, assessing their expression efficacy and the protective immunity generated by LNP-encapsula...
et al. Lipopolyplex potentiates anti-tumor immunity of mRNA-based vaccination. Biomaterials 125, 81–89 (2017). CAS Google Scholar Zhang, J., Shrivastava, S., Cleveland, R. O. & Rabbitts, T. H. Lipid-mRNA nanoparticle designed to enhance intracellular delivery mediated by shock waves. ...
lipid nanoparticle–mRNA formulations based on zwitterionic ionizable lipids can escape the endosome, leading to efficient protein expression and genome editing in vivo114. In addition to functioning as a delivery component, lipids can have therapeutic effects synergistic with mRNA-encoded proteins117,118...
Here, we investigated factors of importance for lipid nanoparticle (LNP)-mediated delivery of mRNA to the respiratory tract. We hypothesized that: (i) the PEG-lipid content is important for providing colloidal stability during aerosolization and for mucosal delivery, (ii) the PEG-lipid content ...
Ionizable Lipid Nanoparticle-Mediated mRNA Delivery for Human CAR T Cell Engineering. Nano Letters(IF9.6)Pub Date : 2020-02-05, DOI:10.1021/acs.nanolett.9b04246 Margaret M Billingsley1, Nathan Singh2, 3, Pranali Ravikumar2, Rui Zhang1, Carl H June4, 5, Michael J Mitchell1, 4, 6, ...
Many materials have been developed that can be used for mRNA delivery, and notably, lipid nanoparticles are excellent nanodrug carriers for mRNA delivery during cancer im- munotherapy, which is a hot topic in tumor therapy at present [23]. A number of lipid nanoparticle–mRNA formulations for ...
Middle: EpCAM-CD3-hFc mRNA-LNP-treated T cells significantly decrease tumor growth. Right: Tumor size and weight are significantly decreased with EpCAM-CD3-hFc mRNA-LNP-treated T cells. See all the data in the paper “mRNA-Lipid Nanoparticle (LNP) Delivery of Humanized EpCAM-CD3 Bispecific ...
mediated mRNA delivery, we report here a new multiomic nanoparticle delivery system called single-cell nanoparticle targeting-sequencing (SENT-seq). SENT-seq quantifies how dozens of LNPs deliver DNA barcodes and mRNA into cells, the subsequent protein production and the transcriptome, with single-...