Recent Progress in Light Sheet Microscopy for Biological Applications Krishnendu Chatterjee, Feby Wijaya...
Light-sheet microscopy at high resolution.An improved light-sheet microscope images live cells at sub-100-nm axial resolution.doi:10.1038/s41587-021-01101-4Reto FiolkaNature Publishing Group USNature biotechnology
Light-sheet microscopy uses a thin plane of light to illuminate a sample orthogonally to the detection objective such that the axial resolution of the microscope is determined wholly or partly by the thickness of the light sheet. It allows fast volumetric imaging with reduced sample irradiation comp...
Single plane illumination microscopy (SPIM) allows rapid imaging of large, three-dimensional, samples of living tissue. The thin light sheet ensures high contrast whilst photo-bleaching and damage are kept to a minimum. However, many specimen of interest have a significant thickness. To date, hig...
light sheet microscopy tiling light sheet microscopy tiling light sheet selective plane illumination microscopy tissue clearing tissue expansion cleared tissue imaging Introduction High-resolution 3D fluorescence imaging of biological tissues bridges the study of gene expression, cell morphology, and cell distri...
We demonstrate capabilities of SPED microscopy by performing fast sub-cellular resolution imaging of CLARITY mouse brains and cellular-resolution volumetric Ca2+ imaging of entire zebrafish nervous systems. Together, SPED light sheet methods enable high-speed cellular-resolution volumetric mapping of ...
Light-sheet microscopy is the only modality that images the entire organism at cellular resolution in three-dimensions, for the time-span over which migration occurs. Visualization and analysis of the light-sheet data will be facilitated by the use of Diderot, a new parallel domain-specific ...
light sheet microscopy techniques that rely on fast framerates such as SCAPE or lattice light sheet microscopy will be able to deliver more than ever before. For larger samples, the 29.4 mm square sensor of the Kinetix is designed to increase throughput, maximize the amount of data captured in...
Push the boundaries of research with reliable, precise and versatile light sheet microscopy solutions from Leica Microsystems to study developing embryos, spheroids, organoids or other delicate samples. Intestinal organoids label with FUCCI reporter to follow cell cycle dynamics. Courtesy of Franziska Moo...
在这里,我们展示了NuMorph(基于核的形态测量),一组分析工具,用于量化出生后第4天(P4)小鼠大脑注释区域内的所有细胞核和核标记物,在光片显微镜上清除和成像。我们描述了磁共振成像(MRI),以在组织清除脱水步骤引起的收缩之前测量脑体积,使用iDISCO+方法清除组织,包括免疫标记,然后使用市售平台进行光片显微镜...