Light-sheet microscopy obtains optical sections by illuminating the specimen with sheet-shaped excitation light(s) from the side. This method has great adv... S Nonaka - 《Kenbikyo》 被引量: 0发表: 2012年 Scanned light sheet microscopy with confocal slit detection Thus the imaging principle pr...
cleared samples in a free and open-source way. The mesoSPIM system achieves uniform axial resolution across cm-scale FOV by using the axially swept light-sheet microscopy principle (ASLM)33,34,35,36. In brief, the axially most confined region of the light-...
Selective plane illumination microscopy (SPIM a type of light-sheet microscopy) involves focusing a thin sheet of laser light through a specimen at right angles to the objective lens. As only the thin section of the specimen at the focal plane of the lens is illuminated, out of focus light...
Light-sheet fluorescence microscopy (LSFM) has become an important tool for biological and biomedical research. Although several illumination and detection strategies have been developed, the sample mounting still represents a cumbersome procedure as this is highly dependent on the type of sample and oft...
light sheet microscopy tiling light sheet microscopy tiling light sheet selective plane illumination microscopy tissue clearing tissue expansion cleared tissue imaging Introduction High-resolution 3D fluorescence imaging of biological tissues bridges the study of gene expression, cell morphology, and cell distri...
LSFM : Light Sheet Fluorescence Microscopy NA : Numeric Aperture PBRT : Physically-based Rendering Toolkit PCM : Phase Contrast Microscopy PSF : Point Spread Function RFP : Red Fluorescent Protein RTE : Radiative Transfer Equation SPD : Spectral Power Distribution.References...
Optical microscopy further confirmed the consistent and uniform distribution of the recording sites. We used double-sided polyimide tape to affix the flexible probe onto the PCB substrate, followed by the application of solder across all pads and vias. The Intan chip, which amplifies, filters, ...
phase modulation; lattice light sheet microscopy; digital holography; fluorescence microscopy1. Introduction Excitable cells, whether neurons or muscle cells, rapidly transport ions during excitation [1]. Excitation may be due to direct mechanical deformation in mechanoreceptive cells [2], or following,...
Figure 3.Staphylococcus aureus growth on selective medium: (a) sample on raw milk; (b) sample on milk from a cow treated for mastitis; (c) Gram smear microscopy on sample b. According to the research results, it was found that the total microbial count in raw milk was within the specif...
An introduction to light-sheet microscopy, an imaging technique where a sheet of laser light illuminates only a thin slice of live or cleared samples.